Human/Mouse/Rat PTP mu/PTPRM Antibody

Catalog #: MAB4446
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4446
MAB4446-SP

Detection of Human PTPμ/PTPRM by Western Blot.

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All PTP mu/PTPRM Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat PTP mu/PTPRM Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat PTPμ/PTPRM in Western blots. In Western blots, no cross-reactivity with recombinant human PTPRK, PTPRT, PTPRD, PTPRG, PTPRF (LAR), or DEP1 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 436502

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human PTPμ/PTPRM
Glu21-Lys742
Accession # P28827

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human PTPμ/PTPRM by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, and CHP-100 human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat PTPµ/PTPRM Monoclonal Antibody (Catalog # MAB4446) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PTPµ/PTPRM at approximately 280 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PTP mu/PTPRM

Protein Tyrosine Phosphatase, receptor type M (PTPRM), also called PTPμ, PTPRμ, and RPTPμ, is expressed at highest levels in pulmonary vascular epithelia, where interactions with cadhedrins are believed to be important in regulating barrier permeability. The MAM and Fibronectin III (FNIII) domains on the extracellular side of PTPRM bind between cells, affecting adhesion and contact inhibition. Culturing cells to high density concentrates PTPRM at sites of tight contact and induces proteolytic cleavage of the 100 kDa extracellular domain.

Long Name

Protein Tyrosine Phosphatase Receptor-type M

Entrez Gene IDs

5797 (Human); 19274 (Mouse); 29616 (Rat)

Alternate Names

EC 3.1.3.48; hR-PTPu; MGC166994; protein tyrosine phosphatase mu; protein tyrosine phosphatase, receptor type, M; protein tyrosine phosphatase, receptor type, mu polypeptide; Protein-tyrosine phosphatase mu; PTP mu; PTPRL1; PTPRL1R-PTP-MU; PTPRM; receptor-type tyrosine-protein phosphatase mu; RPTPM; R-PTP-mu; RPTPU

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human/Mouse/Rat PTP mu/PTPRM Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1