Human/Mouse/Rat Rap1A/B Antibody

Catalog #: AF3767
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3767
AF3767-SP

Detection of Human Rap1A/B by Western Blot.

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All Rap1A/B Antibodies

Product Details

Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Rap1A/B Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse and rat Rap1A/B. The antibody is highly specific for Rap1A/B and detects recombinant human Rap2A only upon prolonged film exposure.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Rap1A
Met1-Leu184
Accession # P62834

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

Immersion fixed paraffin-embedded sections of human skin and human squamous cell carcinoma tissue

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Rap1A/B by Western Blot. Western blot shows recombinant human Rap1A, Rap1B, Rap2A, and Rap2B (1 ng/lane). PVDF membrane was probed with 1 µg/mL Human/Mouse/Rat Rap1A/B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3767) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot

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Detection of Human/Mouse/Rat Rap1A/B by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, MDA-MB-468 human breast cancer cell line, PT18 mouse mast/basophil cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL Human/Mouse/Rat Rap1A/B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3767) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band for Rap1A/B was detected at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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Detection of Rap1A/B in Human Squamous. Rap1A/B was detected in immersion fixed paraffin-embedded sections of Human Squamous using Goat Anti-Human/Mouse/Rat Rap1A/B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3767) at 15 µg/mL for 1 hour at room temperature followed by incubation with HRP-DAB. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Rap1A/B

The Ras-related proteins Rap1A and Rap1B share 95% identity and together with Rap2A and Rap2B constitute a grouping within the Ras superfamily of small GTPases. Like other GTPases, Rap proteins transduce signals by cycling between an active GTP-bound and an inactive GDP-bound state. Rap1 counteracts the mitogenic function of Ras, possibly through competitive interactions with Ras GAPs and Raf, and is implicated in the regulation of integrins.

Long Name

Ras-related Protein 1A/B

Alternate Names

RAP1A; Rap1A/B; RAP1B

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human/Mouse/Rat Rap1A/B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Epstein-Barr virus subverts mevalonate and fatty acid pathways to promote infected B-cell proliferation and survival
Authors: LW Wang, Z Wang, I Ersing, L Nobre, R Guo, S Jiang, S Trudeau, B Zhao, MP Weekes, BE Gewurz
PLoS Pathog., 2019-09-13;15(9):e1008030.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot