Human/Mouse/Rat RKIP Antibody

Name: Human/Mouse/Rat RKIP Antibody
Brand: R&D Systems
SKU: AF4538
Rating: 3.8 (4 reviews)

Catalog #: AF4538 4 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4538
AF4538-SP

Detection of Human/Mouse/Rat RKIP by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat RKIP Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects endogenous human, mouse, and rat RKIP in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human RKIP
Met1-Lys187
Accession # P30086

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat RKIP by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells and A172 human glioblastoma cell line. PVDF membrane was probed with 0.2 µg/mL of Human/Mouse/Rat RKIP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4538) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for RKIP at approximately 23 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RKIP

Raf kinase inhibitor protein (RKIP) is a member of the phosphatidylethanolamine-binding protein (PEBP) family, and modulates the activation of several signal transduction cascades. By binding the MAP3K Raf-1, unphosphorylated RKIP inhibits activation of the MAP2K MEK. PKC-phosphorylated RKIP dissociates from Raf‑1 and inhibits GRK2, resulting in sustained G-protein signaling. Expressed in many tissues, RKIP is implicated in the regulation of such physiological processes as membrane biosynthesis, spermatogenesis, neural development, and metastasis suppression.

Long Name

Raf kinase inhibitory protein

Entrez Gene IDs

5037 (Human); 23980 (Mouse); 29542 (Rat)

Alternate Names

HCNP; HCNPpp; hippocampal cholinergic neurostimulating peptide; Neuropolypeptide h3; PBP; PBPPEBP-1; PEBP1; PEBP-1; PEBPHCNPpp; phosphatidylethanolamine binding protein 1; phosphatidylethanolamine-binding protein 1; prostatic binding protein; Prostatic-binding protein; Raf kinase inhibitory protein; RKIP; RKIPRaf kinase inhibitor protein