Human/Mouse/Rat ROCK1 Antibody

Name: Human/Mouse/Rat ROCK1 Antibody
Brand: R&D Systems
SKU: MAB4590
Rating: 5 (1 reviews)

Catalog #: MAB4590 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4590
MAB4590-SP

Detection of Human, Mouse, and Rat ROCK1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat ROCK1 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human ROCK1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human ROCK2 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 701118

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human ROCK1
Ala629-Thr829
Accession # Q13464

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat ROCK1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, C2C12 mouse myoblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ROCK1 Monoclonal Antibody (Catalog # MAB4590) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ROCK1 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ROCK1

ROCK1 is a ubiquitously expressed serine/threonine kinase that is a downstream target of the small GTPase RhoA. ROCK1 is involved in diverse cellular functions, including smooth muscle contraction, actin cytoskeleton organization, cell adhesion and motility, and gene expression (1). ROCK1 contributes to the development of cardiac fibrosis and induction of fibrogenic cytokines in cardiomyocytes in response to pathological stimuli. ROCK1 knockout mice exhibit reduced perivascular and interstitial fibrosis, which is associated with reduced expression of a variety of extracellular matrix (ECM) proteins and fibrogenic cytokines (2).

References

Zhao, Y.M. et al. (2004) Dev. Biol. 275:183.
Zhang, C. et al. (2006) FASEB J. 20:916.

Long Name

Rho-associated, Coiled-Coil Containing Protein Kinase 1

Entrez Gene IDs

6093 (Human); 19877 (Mouse); 81762 (Rat)

Alternate Names

EC 2.7.11; EC 2.7.11.1; MGC131603; MGC43611; p160 ROCK-1; p160ROCK; p160-ROCK; PRO0435; Renal carcinoma antigen NY-REN-35; Rho kinase; rho-associated protein kinase 1; Rho-associated, coiled-coil containing protein kinase 1; Rho-associated, coiled-coil-containing protein kinase 1; ROCK1; ROK beta