Human/Mouse/Rat ROCK2 Antibody

Catalog #: AF4790
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4790
AF4790-SP

Detection of Human/Mouse/Rat ROCK2 by Western Blot.

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Human/Mouse/Rat ROCK2 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse and rat ROCK2 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human ROCK2
Ile641-Ala846
Accession # O75116

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat ROCK2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, DA3 mouse myeloma cell line, CH-1 mouse B cell lymphoma cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat ROCK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4790) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for ROCK2 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ROCK2

ROCK2/II (Rho-associated protein kinase 2; also Rho-kinase alpha ) is a member of the AGC serine/threonine protein kinase family. ROCK2 is a downstream target of activated RhoA. Its activation results in myosin phosphorylation with subsequent muscle contraction. Human ROCK2 is 1388 amino acids (aa) in length, and appears to be present as a dimer. It contains a protein kinase domain (aa 92‑354), a coiled-coil region (aa 429‑1131) and a zinc-finger region (aa 1260‑1315). Two isoforms show an alternate start site at M686, and a three aa substitution for the N-terminal 444 amino acids. Over aa 641‑846, human ROCK2 shares 94% and 96% aa identity with mouse and canine ROCK2, respectively.

Long Name

Rho-associated, Coiled-coil Containing Protein Kinase 2

Entrez Gene IDs

9475 (Human); 19878 (Mouse); 25537 (Rat)

Alternate Names

coiled-coil-containing protein kinase 2; EC 2.7.11; EC 2.7.11.1; KIAA0619; p164 ROCK-2; Rho kinase 2; rho-associated protein kinase 2; Rho-associated, coiled-coil containing protein kinase 2; ROCK2; ROCK-II; ROKalpha

Product Datasheets

Product Datasheet

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Related Research Areas

Citation for Human/Mouse/Rat ROCK2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1