Human/Mouse/Rat SHP-2 Antibody

Catalog #: AF1894 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1894
AF1894-SP

Detection of Human/Mouse/Rat SHP‑2 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat SHP-2 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat SHP-2 in Western blots. Does not detect SHP-1 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human SHP-2
Thr2-Arg593
Accession # NP_002825

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

See below

Knockout Validated

SHP‑2 is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in SHP‑2 knockout HEK293T cell line.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat SHP‑2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, HT-29 human colon adenocarcinoma cell line, Raji human Burkitt's lymphoma cell line, and NR-6 mouse fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL Goat Anti-Human/Mouse/Rat SHP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1894) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, Recombinant Human Active SHP-1 aa 205-595 (Catalog # 1878-SH) and Recombinant Human SHP-2 (Catalog # 1894-SH) (2 ng/lane) were included. A specific band for SHP-2 was detected at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Knockout Validated

Western Blot Shows Human SHP-2 Antibody Specificity by Using Knockout Cell Line.

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Western Blot Shows Human SHP‑2 Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and SHP-2 knockout HEK293T cell line (KO). PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse/Rat SHP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1894) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SHP-2 at approximately 80 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SHP-2

Src-Homology domain-2 containing protein tyrosine Phosphatase 2 (SHP-2), also called protein tyrosine phosphatase, non-receptor type 11 (PTPN11), PTP1D, PTP2C, and SYP, is an enzyme that dephosphorylates tyrosine residues in proteins. The protein contains two Src homology 2 (SH2) domains, which both regulate the activity of the enzyme (1) and allow it to selectively bind to SH2 sites on proteins such as Dok1, IRS1, and the insulin receptor (2). SHP-2 plays a unique stimulatory role in cell signaling. Cells lacking SHP-2 have poor mobility because the hyper-phosphorylation of FAK and other proteins in the focal adhesion complex (3) prevents turnover of cellular attachment points. Without SHP-2, sustained ERK stimulation does not take place (4). The Y992 phosphorylation site of EGFR is a particularly good substrate for SHP-2 (5) and a phosphopeptide containing this sequence can be used to measure the activity of the enzyme (R&D Systems, Catalog # ES006) by detecting release of phosphate (R&D Systems, Catalog # DY996).

References

Zhao, Z. et al. (1994) J. Biol. Chem. 269:8780.
Clemmons, D.R. and Maile, L.A. (2005) Mol. Endocrinol. 19:1.
von Wichert, G. et al. (2003) EMBO J. 22:5023.
Maroun, C.R. et al. (2000) Mol. Cell. Biol. 20:8513.
Sugimoto, S. et al. (1993) J. Biol. Chem. 269:22771.

Long Name

Src Homology Region 2 Domain Phosphatase 2

Entrez Gene IDs

5781 (Human); 54390 (Mouse); 25622 (Rat)

Alternate Names

BPTP3; EC 3.1.3.48; MGC14433; protein tyrosine phosphatase, non-receptor type 11; protein tyrosine phosphatase-2; Protein-tyrosine phosphatase 1D; Protein-tyrosine phosphatase 2C; PTP1D; PTP-1D; PTP2C; PTP-2C; PTP2CNS1; PTPN11; SHP2; SHP-2; SHP2CFC; SHPTP2; SH-PTP2; SH-PTP2Noonan syndrome 1; SH-PTP3; tyrosine-protein phosphatase non-receptor type 11