Home / STAT6 / Human/Mouse/Rat STAT6 aa 627-838 Antibody

Human/Mouse/Rat STAT6 aa 627-838 Antibody

Catalog #: AF2167
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2167
AF2167-SP
Detection of Human STAT6 by Western Blot.
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Product Details
Citations (4)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat STAT6 aa 627-838 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat STAT6 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse STAT6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Daudi human Burkitt's lymphoma cell line
Simple Western
10 µg/mL
Daudi human Burkitt's lymphoma cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot View Larger

Detection of Human STAT6 by Western Blot. Western Blot shows lysates of Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/ml of Goat Anti-Human/Mouse/Rat STAT6 aa 627-838 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2167) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for STAT6 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Intracellular Staining by Flow Cytometry Detection of STAT6 antibody in HeLa Human Cell Line antibody by Flow Cytometry. View Larger

Detection of STAT6 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Goat Anti-Human/Mouse/Rat STAT6 aa 627-838 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2167, filled histogram) or control antibody (AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.

Simple Western View Larger

Detection of Human STAT6 by Simple WesternTM. Simple Western shows lysates of Daudi human Burkitt's lymphoma cell line, loaded at 0.5 mg/ml. A specific band was detected for STAT6 at approximately 98 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat STAT6 aa 627-838 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2167). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: STAT6

Signal Transducer and Activator of Transcription 6 (STAT6) is a member of the Jak/STAT signal transduction pathway and mediates cytokine signaling by IL-4. IL-4 activation leads to tyrosine phosphorylation and accumulation in the nucleus of STAT6 homo and heterodimers. STAT6 acts as a signal transducer in the cytoplasm and, upon phosphorylation at Y641, translocates to the nucleus and binds to the DNA consensus site TTCN4GAA. Knockout studies in mice suggest that STAT6 functions in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins. STAT6 transcriptional activation occurs by binding p100 and p160 class (NcoA1, Src-1) co-activators. Gene expression studies indicate that STAT6 mediates cell proliferation and anti-apoptotic effects of IL-4.

Long Name
Signal Transducer and Activator of Transcription 6
Entrez Gene IDs
6778 (Human); 20852 (Mouse); 362896 (Rat)
Alternate Names
D12S1644; EC 2.4.1.227; EC 2.7.7.6; IL-4 Stat; IL-4-STAT; signal transducer and activator of transcription 6; signal transducer and activator of transcription 6, interleukin-4 induced; STAT, interleukin-4 induced; STAT, interleukin4-induced; STAT6; STAT6B; STAT6C; transcription factor IL-4 STAT

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Citations for Human/Mouse/Rat STAT6 aa 627-838 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Periostin Activation of Integrin Receptors on Sensory Neurons Induces Allergic Itch
    Authors: SK Mishra, JJ Wheeler, S Pitake, H Ding, C Jiang, T Fukuyama, JS Paps, P Ralph, J Coyne, M Parkington, J DeBrecht, LC Ehrhardt-H, GP Cruse, W Bäumer, RR Ji, MC Ko, T Olivry
    Cell Rep, 2020-04-07;31(1):107472.
  2. Lactate dehydrogenase A regulates tumor-macrophage symbiosis to promote glioblastoma progression
    Authors: Khan, F;Lin, Y;Ali, H;Pang, L;Dunterman, M;Hsu, WH;Frenis, K;Grant Rowe, R;Wainwright, DA;McCortney, K;Billingham, LK;Miska, J;Horbinski, C;Lesniak, MS;Chen, P;
    Nature communications
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. LDHA-regulated tumor-macrophage symbiosis promotes glioblastoma progression
    Authors: Chen, P;Khan, F;Lin, Y;Ali, H;Pang, L;Dunterman, M;Hsu, WH;Frenis, K;Rowe, RG;Wainwright, D;McCortney, K;Billingham, L;Miska, J;Horbinski, C;Lesniak, M;
    Research square
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Absence of Tumor Necrosis Factor Supports Alternative Activation of Macrophages in the Liver after Infection with
    Authors: S Hu, C Marshall, J Darby, W Wei, AB Lyons, H Körner
    Front Immunol, 2018-01-19;9(0):1.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Western Blot

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