Human/Mouse ROR gamma t/RORC2/NR1F3 PE-conjugated Antibody
Human/Mouse ROR gamma t/RORC2/NR1F3 PE-conjugated Antibody Summary
Met1-Arg10
Accession # P51449
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of ROR gamma t/RORC2/NR1F3 in CD4+/CD8+Mouse Thymocytes by Flow Cytometry. CD4+/CD8+mouse thymocytes were stained with Mouse Anti-Human/Mouse ROR gammat/RORC2/NR1F3 PE-conjugated Monoclonal Antibody (Catalog # IC6006P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Detection of ROR gamma t/RORC2/NR1F3 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with PMA, Calcium Ionomycin, LPS, and Recombinant Human IL-23 (Catalog # 1290-IL) were stained with Mouse Anti-Human/Mouse ROR gammat/RORC2/ NR1F3 PE-conjugated Monoclonal Antibody (Catalog # IC6006P) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC0041P). To facilitate intra-cellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: ROR gamma t/RORC2/NR1F3
Retinoic acid-related Orphan Receptor gamma (ROR gamma, TOR, RORC; NR1F3) is a member of the orphan nuclear receptor family. ROR gamma is expressed in the muscle, thymus, testis, pancreas, prostate, heart, and liver. ROR gamma plays a role in thymocyte development and homeostasis. RORs bind to DNA as monomers on half-site elements with 5' A/T-rich extensions. An N-terminal isoform of ROR gamma, ROR gamma t, has been shown to be specifically expressed in the thymus.
Product Datasheets
Citations for Human/Mouse ROR gamma t/RORC2/NR1F3 PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Vitamin D3 and zinc synergistically induce regulatory T cells and suppress interferon-gamma production in mixed lymphocyte culture
Authors: AK Schmitt, MA Puppa, I Wessels, L Rink
The Journal of nutritional biochemistry, 2022-01-19;0(0):108942.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Itch inhibits IL-17-mediated colon inflammation and tumorigenesis by ROR-gammat ubiquitination.
Authors: Kathania M, Khare P, Zeng M, Cantarel B, Zhang H, Ueno H, Venuprasad K
Nat Immunol, 2016-06-20;17(8):997-1004.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Pathogen-induced human TH17 cells produce IFN-gamma or IL-10 and are regulated by IL-1beta.
Authors: Zielinski CE, Mele F, Aschenbrenner D, Jarrossay D, Ronchi F, Gattorno M, Monticelli S, Lanzavecchia A, Sallusto F
Nature, 2012-04-26;484(7395):514-8.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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