Human/Mouse sFRP-3 Antibody

Catalog #: MAB192 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB192-050
MAB192-500
MAB192-SP

All sFRP-3 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse sFRP-3 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human sFRP-3 in direct ELISAs and Western blots. Shows 100% cross-reactivity with recombinant mouse (rm) sFRP‑3 and no cross-reactivity with recombinant human (rh) sFRP-1, rmsFRP-2, or rhsFRP-4.

Source

Monoclonal Mouse IgG₁ Clone # 133912

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human sFRP-3
Ala33-Asn325
Accession # AAB51298

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human sFRP-3 (Catalog # 192-SF)
Recombinant Mouse sFRP-3 (Catalog # 592-FR)
under non-reducing conditions only

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: sFRP-3

sFRP-3, also known as FRZB, Fritz, Frzb1 and FRP-3, is expressed in the developing bone and cartilage as well as several adult tissues. It functions as an antagonist of Wnt signaling.

Long Name

Secreted Frizzled Related Protein 3

Entrez Gene IDs

2487 (Human); 20378 (Mouse)

Alternate Names

FIZ; FREOS1; Frezzled; Fritz; Frizzled-related protein 1; frizzled-related protein; FRP; FRP-3; FRZB; Frzb1; FRZB-1; FRZB1sFRP-3; FRZB-PEN; FZRB; hFIZ; secreted frizzled-related protein 3; sFRP3; sFRP-3; SFRP3frezzled; SRFP3frizzled homolog-related