Human/Mouse sFRP-3 Biotinylated Antibody

Catalog #: BAM192 Datasheet / COA / SDS

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BAM192

All sFRP-3 Antibodies

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Human/Mouse sFRP-3 Biotinylated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human sFRP-3 in ELISAs. In sandwich immunoassays, 100% cross-reactivity with recombinant mouse sFRP-3 is observed and no cross-reactivity with recombinant human sFRP-1, -2, or -4 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 133912

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human sFRP-3
Ala33-Asn325
Accession # AAB51298

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Human/Mouse sFRP-3 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.5-2.0 µg/mL

Use in combination with:

Capture Reagent: Human/Mouse sFRP‑3 Antibody (Catalog # MAB1921)

Standard: Recombinant Human sFRP-3 Protein (Catalog # 192-SF)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: sFRP-3

Secreted Frizzled-related protein (sFRP-3), also known as FRZB, Fritz, Frzb1 and FRP-3, is expressed in the developing bone and cartilage as well as several adult tissues. It functions as an antagonist of Wnt signaling.

Long Name

Secreted Frizzled Related Protein 3

Entrez Gene IDs

2487 (Human); 20378 (Mouse)

Alternate Names

FIZ; FREOS1; Frezzled; Fritz; Frizzled-related protein 1; frizzled-related protein; FRP; FRP-3; FRZB; Frzb1; FRZB-1; FRZB1sFRP-3; FRZB-PEN; FZRB; hFIZ; secreted frizzled-related protein 3; sFRP3; sFRP-3; SFRP3frezzled; SRFP3frizzled homolog-related