Home / SSEA-4 / Human/Mouse SSEA-4 Fluorescein-conjugated Antibody

Human/Mouse SSEA-4 Fluorescein-conjugated Antibody

Catalog #: FAB1435F
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB1435F-100
FAB1435F-025
Detection of SSEA‑4 in NTera‑2 human testicular embryonic carcinoma cell line by Flow Cytometry.
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Citations (5)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse SSEA-4 Fluorescein-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Recognizes a carbohydrate epitope of SSEA-4 (1, 2).
Source
Monoclonal Mouse IgG3 Clone # MC-813-70
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
2120Ep human embryonal carcinoma cell line
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of SSEA-4 antibody in NTera-2 human testicular embryonic carcinoma cell line antibody by Flow Cytometry. View Larger

Detection of SSEA‑4 in NTera‑2 human testicular embryonic carcinoma cell line by Flow Cytometry. NTera-2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/Mouse SSEA-4 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1435F, filled histogram) or isotype control antibody (Catalog # IC007F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: SSEA-4

SSEA-4 is expressed on the surface of human embryonal carcinoma (EC) cells (the pluripotent stem cells of teratocarcinomas), human embryonic germ cells (EG), and human embryonic stem cells (ES). Expression of SSEA-4 is down-regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression (1-4).

References
  1. Shevinsky, L.H. et al. (1982) Cell 30:697.
  2. Kannagi, R. et al. (1983) EMBO J. 2:2355.
  3. Thomson, J.A. and J.S. Odorico (2000) Trends Biotechnol. 18:53.
  4. Draper, J.S. et al. (2002) J. Anat. 200:249.
Long Name
Stage-specific Embryonic Antigen-4
Alternate Names
SSEA4; SSEA-4; SSEA-4;stage-specific embryonic antigen 4

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Citations for Human/Mouse SSEA-4 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Generation of the induced human pluripotent stem cell lines CSSi009-A from a patient with a GNB5 pathogenic variant, and CSSi010-A from a CRISPR/Cas9 engineered GNB5 knock-out human cell line
    Authors: N Malerba, P Benzoni, GM Squeo, R Milanesi, F Giannetti, LG Sadleir, G Poke, B Augello, AI Croce, A Barbuti, G Merla
    Stem Cell Res, 2019-08-22;40(0):101547.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Adequate taylor couette flow-mediated shear stress is useful for dissociating human iPS cell-derived cell aggregates
    Authors: K Matsuura, M Wada, K Sakaguchi, Y Matsuhashi, T Shimizu
    Regen Ther, 2019-04-25;12(0):6-13.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Derivation of human induced pluripotent stem cell line EURACi004-A from skin fibroblasts of a patient with Arrhythmogenic Cardiomyopathy carrying the heterozygous PKP2 mutation c.2569_3018del50
    Authors: B Ermon, CB Volpato, G Cattelan, R Silipigni, M Di Segni, C Cantaloni, M Casella, PP Pramstalle, G Pompilio, E Sommariva, V Meraviglia, A Rossini
    Stem Cell Res, 2018-09-06;32(0):78-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Generation of human induced pluripotent stem cells (EURACi001-A, EURACi002-A, EURACi003-A) from peripheral blood mononuclear cells of three patients carrying mutations in the CAV3 gene
    Authors: V Meraviglia, P Benzoni, S Landi, C Murano, M Langione, BM Motta, S Baratto, R Silipigni, M Di Segni, PP Pramstalle, D DiFrancesc, E Gazzerro, A Barbuti, A Rossini
    Stem Cell Res, 2017-12-18;27(0):25-29.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Fibroblast growth factor-2 maintains a niche-dependent population of self-renewing highly potent non-adherent mesenchymal progenitors through FGFR2c.
    Authors: Di Maggio N, Mehrkens A, Papadimitropoulos A, Schaeren S, Heberer M, Banfi A, Martin I
    Stem Cells, 2012-07-01;30(7):1455-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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