Human/Mouse THAP11 Antibody

Catalog #: MAB5727 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5727
MAB5727-SP

Detection of Human THAP11 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse THAP11 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse THAP11 in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 605383

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived THAP11
Gly226-Met314
Accession # Q96EK4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Simple Western

20 µg/mL

See below

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human THAP11 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse THAP11 Monoclonal Antibody (Catalog # MAB5727) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for THAP11 at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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THAP11 in BG01V Human Stem Cells. THAP11 was detected in immersion fixed undifferentiated BG01V human embryonic stem cells using Mouse Anti-Human/Mouse THAP11 Monoclonal Antibody (Catalog # MAB5727) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western

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Detection of Human THAP11 by Simple Western^TM. Simple Western lane view shows lysates of BG01V human embryonic stem cells, loaded at 0.2 mg/mL. A specific band was detected for THAP11 at approximately 56 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse THAP11 Monoclonal Antibody (Catalog # MAB5727). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: THAP11

THAP11, also known as Ronin, is a 36-44 kDa member of the thanatos-associated protein family. It is ubiquitously expressed and through its repression of c-Myc transcription, blocks cell proliferation. Human THAP11 is 314 amino acids (aa) in length. It contains a DNA-binding THAP zinc-finger domain (aa 5-81), an NLS (aa 98-104), a polyGln region (aa 104-146), an Ala-rich segment (aa 197-221) and a C-terminal coiled-coil region (aa 255-305). THAP11 is phosphorylated on Thr25. Over aa 226-314, human and mouse THAP11 are 100% identical in amino acid sequence.

Long Name

THAP Domain Containing 11

Entrez Gene IDs

57215 (Human); 59016 (Mouse); 307806 (Rat)

Alternate Names

CTG-B43a; CTG-B45d; HRIHFB2206; Ronin; THAP domain containing 11; THAP domain-containing protein 11; THAP11