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Human/Mouse UCP1 Antibody

Catalog #: MAB6158
28 Citations 2 Reviews Datasheet / COA / SDS
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MAB6158
MAB6158-SP
Detection of Human UCP1 by Western Blot.
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Product Details
Citations (28)
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Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse UCP1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse UCP1 in Western blots.
Source
Monoclonal Mouse IgG2B Clone # 536435
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human UCP1
Met1-Thr307
Accession # P25874
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
2.5 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human UCP1 antibody by Western Blot. View Larger

Detection of Human UCP1 by Western Blot. Western blot shows recombinant human UCP1, recombinant human UCP2, recombinant human UCP3, and recombinant human UCP4 (5 ng/lane). PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse UCP1 Monoclonal Antibody (Catalog # MAB6158) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Western Blot Detection of Mouse UCP1 antibody by Western Blot. View Larger

Detection of Mouse UCP1 by Western Blot. Western blot shows lysates of mouse brown adipose tissue and mouse adipose tissue. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse UCP1 Monoclonal Antibody (Catalog # MAB6158) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for UCP1 at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunocytochemistry UCP1 antibody in Human Mesenchymal Stem Cells by Immunocytochemistry (ICC). View Larger

UCP1 in Human Mesenchymal Stem Cells. UCP1 was detected in immersion fixed human mesenchymal stem cells undifferentiated (lower panel) or differentiated into adipocytes (upper panel) using Mouse Anti-Human/Mouse UCP1 Monoclonal Antibody (Catalog # MAB6158) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Intracellular Staining by Flow Cytometry Detection of UCP1 antibody in 3T3-L1 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of UCP1 in 3T3-L1 Mouse Cell Line by Flow Cytometry. 3T3-L1 mouse embryonic fibroblast adipose-like cell line was stained with Mouse Anti-Human/Mouse UCP1 Monoclonal Antibody (Catalog # MAB6158, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Simple Western Detection of Mouse UCP1 antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Mouse UCP1 by Simple WesternTM. Simple Western lane view shows lysates of mouse adipose tissue and mouse brown adipose tissue, loaded at 0.5 mg/mL. A specific band was detected for UCP1 at approximately 37 kDa (as indicated) using 2.5 µg/mL of Mouse Anti-Human/Mouse UCP1 Monoclonal Antibody (Catalog # MAB6158). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: UCP1

Mitochondrial brown fat uncoupling protein 1 (UCP1; also Thermogenin and UCP) is a 33 kDa member of the mitochondrial carrier family of proteins. Human and mouse UCP1 are both 307 amino acids (aa) in length and contain three solcar repetitive regions and six transmembrane segments. UCP1 is found in brown adipose tissue, where it becomes activated by fatty acids and inhibited by nucleotides. It functions as a mitochondrial transporter that creates a proton leak across the inner mitochondrial membrane, uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. Human and mouse UCP1 share 79% aa sequence identity.

Long Name
Uncoupling Protein 1
Entrez Gene IDs
7350 (Human); 22227 (Mouse); 24860 (Rat)
Alternate Names
SLC25A7; SLC25A7mitochondrial brown fat uncoupling protein 1; Solute carrier family 25 member 7; Thermogenin; UCP 1; UCP; UCP1; uncoupling protein 1 (mitochondrial, proton carrier)

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Citations for Human/Mouse UCP1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

28 Citations: Showing 1 - 10
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  1. Impairment of adrenergically-regulated thermogenesis in brown fat of obesity-resistant mice is compensated by non-shivering thermogenesis in skeletal muscle
    Authors: P Janovska, P Zouhar, K Bardova, J Otahal, M Vrbacky, T Mracek, K Adamcova, L Lenkova, J Funda, T Cajka, Z Drahota, S Stanic, AC Rustan, O Horakova, J Houstek, M Rossmeisl, J Kopecky
    Molecular Metabolism, 2023-01-30;69(0):101683.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Regulation of beige adipocyte thermogenesis by the cold-repressed ER protein NNAT
    Authors: KM Choi, CY Ko, SM An, SH Cho, DJ Rowland, JH Kim, A Fasoli, AJ Chaudhari, DM Bers, JC Yoon
    Molecular Metabolism, 2023-01-25;0(0):101679.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. MYPT1-PP1beta phosphatase negatively regulates both chromatin landscape and co-activator recruitment for beige adipogenesis
    Authors: H Takahashi, G Yang, T Yoneshiro, Y Abe, R Ito, C Yang, J Nakazono, M Okamoto-Ka, A Uchida, M Arai, H Jin, H Choi, M Tumenjarga, S Xie, J Zhang, H Sagae, Y Zhao, R Yamaguchi, Y Nomura, Y Shimizu, K Yamada, S Yasuda, H Kimura, T Tanaka, Y Wada, T Kodama, H Aburatani, MS Zhu, T Inagaki, TF Osborne, T Kawamura, Y Ishihama, Y Matsumura, J Sakai
    Nature Communications, 2022-09-29;13(1):5715.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Chronic Fatty Acid Depletion Induces Uncoupling Protein 1 (UCP1) Expression to Coordinate Mitochondrial Inducible Proton Leak in a Human-Brown-Adipocyte Model
    Authors: Y Takeda, P Dai
    Cells, 2022-06-27;11(13):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. IL-1R-IRAKM-Slc25a1 signaling axis reprograms lipogenesis in adipocytes to promote diet-induced obesity in mice
    Authors: W Liu, H Zhou, H Wang, Q Zhang, R Zhang, B Willard, C Liu, Z Kang, X Li, X Li
    Nature Communications, 2022-05-18;13(1):2748.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Lipocalin 2 Deficiency Alters Prostaglandin Biosynthesis and mTOR Signaling Regulation of Thermogenesis and Lipid Metabolism in Adipocytes
    Authors: J Deis, TY Lin, T Bushman, X Chen
    Cells, 2022-05-03;11(9):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Capsaicin directly promotes adipocyte browning in the chemical compound-induced brown adipocytes converted from human dermal fibroblasts
    Authors: Y Takeda, P Dai
    Scientific Reports, 2022-04-22;12(1):6612.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Progenitor cells from brown adipose tissue undergo neurogenic differentiation
    Authors: M Jumabay, L Zhang, J Yao, KI Boström
    Scientific Reports, 2022-04-04;12(1):5614.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  9. Defective brown adipose tissue thermogenesis and impaired glucose metabolism in mice lacking Letmd1
    Authors: KM Choi, JH Kim, X Kong, M Isik, J Zhang, HW Lim, JC Yoon
    Cell Reports, 2021-12-14;37(11):110104.
    Species: Mouse, Transgenic Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Regulatory modules of human thermogenic adipocytes: functional genomics of large cohort and Meta-analysis derived marker-genes
    Authors: B B Tóth, Z Barta, ÁB Barta, L Fésüs
    BMC Genomics, 2021-12-11;22(1):886.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Latent TGFbeta-binding proteins regulate UCP1 expression and function via TGFbeta2
    Authors: D Halbgebaue, J Roos, JB Funcke, H Neubauer, BS Hamilton, E Simon, EZ Amri, KM Debatin, M Wabitsch, P Fischer-Po, D Tews
    Molecular Metabolism, 2021-09-01;53(0):101336.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. Hepatic AKT orchestrates adipose tissue thermogenesis via FGF21-dependent and -independent mechanisms
    Authors: J Sostre-Col, K Uehara, AE Garcia Whi, MJ Gavin, J Ishibashi, MJ Potthoff, P Seale, PM Titchenell
    Cell Reports, 2021-05-18;35(7):109128.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  13. Brown and beige adipose tissue regulate systemic metabolism through a metabolite interorgan signaling axis
    Authors: A Whitehead, FN Krause, A Moran, ADV MacCannell, JL Scragg, BD McNally, E Boateng, SA Murfitt, S Virtue, J Wright, J Garnham, GR Davies, J Dodgson, JE Schneider, AJ Murray, C Church, A Vidal-Puig, KK Witte, JL Griffin, LD Roberts
    Nature Communications, 2021-03-26;12(1):1905.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  14. Transcriptome analysis reveals brown adipogenic reprogramming in chemical compound-induced brown adipocytes converted from human dermal fibroblasts
    Authors: Y Takeda, T Yoshikawa, P Dai
    Scientific Reports, 2021-03-03;11(1):5061.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. Adult mice are unresponsive to AAV8-Gremlin1 gene therapy targeting the liver
    Authors: R Khatib Sha, J M Hoffmann, S Hedjazifar, L Bonnet, R K Baboota, S Heasman, C Church, I Elias, F Bosch, J Boucher, A Hammarsted, U Smith
    PLoS ONE, 2021-02-19;16(2):e0247300.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  16. Kdm2a deficiency in macrophages enhances thermogenesis to protect mice against HFD-induced obesity by enhancing H3K36me2 at the Pparg locus
    Authors: L Chen, J Zhang, Y Zou, F Wang, J Li, F Sun, X Luo, M Zhang, Y Guo, Q Yu, P Yang, Q Zhou, Z Chen, H Zhang, Q Gong, J Zhao, DL Eizirik, Z Zhou, F Xiong, S Zhang, CY Wang
    Cell Death and Differentiation, 2021-01-18;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  17. 14-3-3? mediates an alternative, non-thermogenic mechanism in male mice to reduce heat loss and improve cold tolerance
    Authors: K Diallo, S Dussault, C Noll, AF Lopez, A Rivard, AC Carpentier, GE Lim
    Mol Metab, 2020-07-12;41(0):101052.
    Species: Mouse
    Sample Types: Protein
    Applications: ICC
  18. Early B Cell Factor Activity Controls Developmental and Adaptive Thermogenic Gene Programming in Adipocytes
    Authors: AR Angueira, SN Shapira, J Ishibashi, S Sampat, J Sostre-Col, MJ Emmett, PM Titchenell, MA Lazar, HW Lim, P Seale
    Cell Rep, 2020-03-03;30(9):2869-2878.e4.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  19. A developed serum-free medium and an optimized chemical cocktail for direct conversion of human dermal fibroblasts into brown adipocytes
    Authors: Y Takeda, P Dai
    Sci Rep, 2020-02-28;10(1):3775.
    Species: Human
    Sample Types: Cell Culture Lysates
    Applications: Western Blot
  20. BMP4 gene therapy enhances insulin sensitivity but not adipose tissue browning in obese mice
    Authors: JM Hoffmann, JR Grünberg, A Hammarsted, T Kroon, TU Greiner, S Maurer, I Elias, V Palsdottir, F Bosch, J Boucher, S Hedjazifar, U Smith
    Mol Metab, 2019-12-17;32(0):15-26.
    Species: Mouse
    Sample Types: Tissue ho
    Applications: Western Blot
  21. Differentiating SGBS adipocytes respond to PPAR? stimulation, irisin and BMP7 by functional browning and beige characteristics
    Authors: Á Klusóczki, Z Veréb, A Vámos, P Fischer-Po, M Wabitsch, Z Bacso, L Fésüs, E Kristóf
    Sci Rep, 2019-04-09;9(1):5823.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  22. Mature Human White Adipocytes Cultured under Membranes Maintain Identity, Function, and Can Transdifferentiate into Brown-like Adipocytes
    Authors: MJ Harms, Q Li, S Lee, C Zhang, B Kull, S Hallen, A Thorell, I Alexanders, CE Hagberg, XR Peng, A Mardinoglu, KL Spalding, J Boucher
    Cell Rep, 2019-04-02;27(1):213-225.e5.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  23. A Renewable Source of Human Beige Adipocytes for Development of Therapies to Treat Metabolic Syndrome
    Authors: S Su, AR Guntur, DC Nguyen, SS Fakory, CC Doucette, C Leech, H Lotana, M Kelley, J Kohli, J Martino, S Sims-Lucas, L Liaw, C Vary, CJ Rosen, AC Brown
    Cell Rep, 2018-12-11;25(11):3215-3228.e9.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  24. Flow Cytometry of Mouse and Human Adipocytes for the Analysis of Browning and Cellular Heterogeneity
    Authors: CE Hagberg, Q Li, M Kutschke, D Bhowmick, E Kiss, IG Shabalina, MJ Harms, O Shilkova, V Kozina, J Nedergaard, J Boucher, A Thorell, KL Spalding
    Cell Rep, 2018-09-04;24(10):2746-2756.e5.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  25. IRX3 Promotes the Browning of White Adipocytes and Its Rare Variants are Associated with Human Obesity Risk
    Authors: Y Zou, P Lu, J Shi, W Liu, M Yang, S Zhao, N Chen, M Chen, Y Sun, A Gao, Q Chen, Z Zhang, Q Ma, T Ning, X Ying, J Jin, X Deng, B Shen, Y Zhang, B Yuan, S Kauderer, S Liu, J Hong, R Liu, G Ning, W Wang, W Gu, J Wang
    EBioMedicine, 2017-09-13;24(0):64-75.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  26. Both brown adipose tissue and skeletal muscle thermogenesis processes are activated during mild to severe cold adaptation in mice
    Authors: NC Bal, S Singh, FCG Reis, SK Maurya, S Pani, LA Rowland, M Periasamy
    J. Biol. Chem., 2017-08-09;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  27. Direct conversion of human fibroblasts to brown adipocytes by small chemical compounds
    Authors: Y Takeda, Y Harada, T Yoshikawa, P Dai
    Sci Rep, 2017-06-27;7(1):4304.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  28. White-to-brown metabolic conversion of human adipocytes by JAK inhibition.
    Authors: Moisan A, Lee Y, Zhang J, Hudak C, Meyer C, Prummer M, Zoffmann S, Truong H, Ebeling M, Kiialainen A, Gerard R, Xia F, Schinzel R, Amrein K, Cowan C
    Nat Cell Biol, 2014-12-08;17(1):57-67.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Reviews for Human/Mouse UCP1 Antibody

Average Rating: 5 (Based on 2 Reviews)

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Human/Mouse UCP1 Antibody
By Matthew Emmett on 07/12/2017
Application: WB Sample Tested: Brown adipose tissue,Primary brown adipocytes- cultured,Primary Brown Adipocytes Species: Mouse

Ucp1 antibody utilized for western blot and tested against Ucp1 KO brown adipose tissue.

Emmett, M. J. et al. Histone deacetylase 3 prepares brown adipose tissue for acute thermogenic challenge. Nature 546, 544-548 (2017).

Human/Mouse UCP1 Antibody
By Aaron Brown on 07/11/2017
Application: WB Sample Tested: Brown adipose tissue Species: Human
Western Blot Human/Mouse UCP1 Antibody MAB6158