Human/Mouse UCP1 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
IC6158P-025
IC6158P-100
Detection of UCP1 in Human Preadipocytes by Flow Cytometry.
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Product Details
Citations (1)
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Human/Mouse UCP1 PE-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse UCP1 in Western blots.
Source
Monoclonal Mouse IgG2B Clone # 536435
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human UCP1
Met1-Thr307
Accession # P25874
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of UCP1 antibody in Human Preadipocytes antibody by Flow Cytometry. View Larger

Detection of UCP1 in Human Preadipocytes by Flow Cytometry. Human preadipocytes were stained with Mouse Anti-Human/Mouse UCP1 PE-conjugated Monoclonal Antibody (Catalog # IC6158P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Intracellular Staining by Flow Cytometry Detection of UCP1 antibody in 3T3-L1 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of UCP1 in 3T3‑L1 Mouse Cell Line by Flow Cytometry. 3T3-L1 mouse embryonic fibroblast adipose-like cell line was stained with Mouse Anti-Human/Mouse UCP1 PE-conjugated Monoclonal Antibody (Catalog # IC6158P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: UCP1

Mitochondrial brown fat uncoupling protein 1 (UCP1; also Thermogenin and UCP) is a 33 kDa member of the mitochondrial carrier family of proteins. Human and mouse UCP1 are both 307 amino acids (aa) in length and contain three solcar repetitive regions and six transmembrane segments. UCP1 is found in brown adipose tissue, where it becomes activated by fatty acids and inhibited by nucleotides. It functions as a mitochondrial transporter that creates a proton leak across the inner mitochondrial membrane, uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. Human and mouse UCP1 share 79% aa sequence identity.

Long Name
Uncoupling Protein 1
Entrez Gene IDs
7350 (Human); 22227 (Mouse); 24860 (Rat)
Alternate Names
SLC25A7; SLC25A7mitochondrial brown fat uncoupling protein 1; Solute carrier family 25 member 7; Thermogenin; UCP 1; UCP; UCP1; uncoupling protein 1 (mitochondrial, proton carrier)

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Citation for Human/Mouse UCP1 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Generation of Functional Brown Adipocytes from Human Pluripotent Stem Cells via Progression through a Paraxial Mesoderm State
    Authors: L Zhang, J Avery, A Yin, AM Singh, TS Cliff, H Yin, S Dalton
    Cell Stem Cell, 2020-08-11;0(0):.
    Species: Human, Xenograft
    Sample Types: Cell Lysates, Whole Cells, Whole Tissue
    Applications: Flow Cytometry, ICC, IHC, Western Blot

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