Human/Mouse WDR5 Antibody

Catalog #: AF5810
5 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5810
AF5810-SP

Detection of Human WDR5 by Western Blot.

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Citations (5)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse WDR5 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse WDR5 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human WDR5
Met1-Lys120
Accession # P61964

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human WDR5 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse WDR5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5810) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for WDR5 was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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WDR5 in Human B-Cell Lymphoma. WDR5 was detected in immersion fixed paraffin-embedded sections of human B-cell lymphoma using Goat Anti-Human/Mouse WDR5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5810) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: WDR5

WDR5 (WD repeat-containing protein 5; also BMP2-induced 3 kb gene protein/BIG3) is a nuclear, 36‑40 kDa monomeric member of the WD family of repeat proteins. It is known to be expressed in osteoblasts, osteocytes and chrondrocytes, among other cells, and appears to serve as a structural organizer for the histone methyltransferase MLL1 complex. This complex methylates histone H3, and within this complex, WDR5 binds to mono- or dimethylated histone H3 and serves as an anchor for complex subunits RbBP5, Ash2L and MLL1. Human WDR5 is 334 amino acids (aa) in length. It contains seven WD domains (aa 43‑333) that likely participate in protein-protein interactions, plus a phosphorylation site at Ser267. There are three isoform variants for WDR5. Human and mouse WDR5 are absolutely identical in aa sequence.

Long Name

WD Repeat-containing Protein 5

Entrez Gene IDs

11091 (Human); 140858 (Mouse); 362093 (Rat)

Alternate Names

BIG3; BIG-3; BMP2-induced 3-kb gene protein; SWD3; SWD3, Set1c WD40 repeat protein, homolog; WD repeat domain 5; WD repeat-containing protein 5; WDR5; WD-repeat protein 5

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse WDR5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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p53 Integrates Temporal WDR5 Inputs during Neuroectoderm and Mesoderm Differentiation of Mouse Embryonic Stem Cells
Authors: Q Li, F Mao, B Zhou, Y Huang, Z Zou, AD denDekker, J Xu, S Hou, J Liu, Y Dou, RC Rao
Cell Rep, 2020-01-14;30(2):465-480.e6.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Expression of WD Repeat Domain 5 (WDR5) is Associated with Progression and Reduced Prognosis in Papillary Thyroid Carcinoma
Authors: W Xu, L Wang, Y An, J Ye
Med. Sci. Monit., 2019-05-20;25(0):3762-3770.
Species: Human
Sample Types: Paraffin-Embedded Tissue
Applications: IHC
PI3K/AKT-mediated upregulation of WDR5 promotes colorectal cancer metastasis by directly targeting ZNF407
Authors: X Tan, S Chen, J Wu, J Lin, C Pan, X Ying, Z Pan, L Qiu, R Liu, R Geng, W Huang
Cell Death Dis, 2017-03-16;8(3):e2686.
Species: Human
Sample Types: Cell Lysates, Whole Tissue
Applications: ChIP, IHC, Western Blot
GRHL3/GET1 and trithorax group members collaborate to activate the epidermal progenitor differentiation program.
Authors: Hopkin, Amelia S, Gordon, William, Klein, Rachel H, Espitia, Francisc, Daily, Kenneth, Zeller, Michael, Baldi, Pierre, Andersen, Bogi
PLoS Genet, 2012-07-19;8(7):e1002829.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
Essential role of the CUL4B ubiquitin ligase in extra-embryonic tissue development during mouse embryogenesis.
Authors: Liu L, Yin Y, Li Y
Cell Res., 2012-03-27;22(8):1258-69.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot