Human Mrc2 APC-conjugated Antibody

Catalog #: FAB5770A Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB5770A-100

Detection of Mrc2 in THP-1 cells by Flow Cytometry.

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Human Mrc2 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Mrc2 in direct ELISA.

Source

Monoclonal Mouse IgG_2B Clone # 106370

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Mouse myeloma cell line, NS0-derived human Mrc2
Gly31-Ala1414
Accession # Q9UBG0

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

THP‑1 human acute monocytic leukemia cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Mrc2 in THP-1 cells by Flow Cytometry. THP-1 cells were stained with Mouse Anti-Human Mrc2 APC‑conjugated Monoclonal Antibody (Catalog # FAB5770A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Mrc2

Mrc2 (C-type Mannose Receptor 2), also know as MMR2, Endocytic Receptor 180 and uPARAP, is a 180‑kDa type I transmembrane protein. It is one of the mannose receptor (MR) family members which share a common domain organization and have a broad range of biological functions (1). Mrc2 is an endocytic receptor that is found on migrating cells, including cancer cells, macrophages, fibroblasts and endothelial cells (2). Mature human Mrc2 is composed of 1449 amino acid (aa) that includes a 1384 aa extracellular domain (ECD), a 21 aa transmembrane region, and a 44 aa cytoplasmic domain. The ECD shows one ricin B‑type lectin domain, one fibronectin type II domain and eight C‑type lectin domains. Within the ECD, human Mrc2 shares 91% aa identity with mouse and rat Mrc2. Mrc2 plays an important role in extracellular matrix remodeling through interaction with its ligands, including Man, Fuc, NAcGlc, collagens and urokinase plasminogen activator receptor (uPAR) (1-3). This cell surface molecule has been reported to promote cell invasion through matrix remodeling by internalizing large fragments of collagen and routing it to the lysosome for intracellular degradation and cell chemotaxis (2). It has also been reported to interact with matrix metalloprotease-13 (MMP-13) and collagen V on the cell surface (4).

References

Yuan, C. et al. (2016) Biochem. J. 473:2359.
Durrel, T. et al. (2018) Nat. Commun. 9:5178.
Behrendt, N. et al. (2000) J. Biol. Chem. 275:1993.
Englehom, L.H. et al. (2001) Lab. Invest. 81:1403.

Long Name

Mannose Receptor, C-type 2

Entrez Gene IDs

9902 (Human); 17534 (Mouse); 498011 (Rat)

Alternate Names

CD280 antigen; CD280; CD280C-type mannose receptor 2; CLEC13E; CLEC13EUPAR-associated protein; Endo180; ENDO180C-type lectin domain family 13 member E; Endocytic receptor 180; KIAA0709FLJ35911; Macrophage mannose receptor 2; mannose receptor, C type 2; Mrc2; uPARAP; UPARAPendocytic receptor (macrophage mannose receptor family); urokinase plasminogen activator receptor-associated protein; Urokinase receptor-associated protein; Urokinase-type plasminogen activator receptor-associated protein