Human MRGPRF Alexa Fluor® 700-conjugated Antibody

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FAB8396N-100UG
R&D Systems Antibodies
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Human MRGPRF Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects HEK293 human embryonic kidney cell line transfected with human MRGPRF by Flow Cytometry. Does not detect untransfected or irrelevant transfected HEK293 cells.
Source
Monoclonal Mouse IgG1 Clone # 916219
Immunogen
NS0 mouse myeloma cell line transfected with human MRGPRF
Met1-Ser343
Accession # NP_659452
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
HEK293 human embryonic kidney cell line transfected with human MRGPRF and eGFP

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MRGPRF

MRGPRF (MAS-related GPR, Member F), also known as RTA, MRGF, GPR140 or GPR168, belongs to the G protein coupled receptor 1 family. MRGPRF may be upregulated during the process of CSF-1 or GM-CSF-induced human monocyte to macrophage differentiation (1).

References
  1. Hohenhaus D.M., et al. (2013) Immunobiology 218:1345.
Long Name
Mas-related G Protein-coupled Receptor Member F
Entrez Gene IDs
116535 (Human); 211577 (Mouse); 266762 (Rat)
Alternate Names
FLJ16111; FLJ29034; FLJ40998; FLJ53714; G protein-coupled receptor 140; G protein-coupled receptor 168; G protein-coupled receptor MrgF; GPR140; GPR168; G-protein coupled receptor 140; G-protein coupled receptor 168; mas-related G protein-coupled MRGF; Mas-related gene F protein; MAS-related GPR, member F; mas-related G-protein coupled receptor member F; MGC21621; mrgF; MRGFDKFZp586B2122; MRGPRF; RTA; seven transmembrane helix receptor

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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