Human MuSK Antibody Summary
Glu22-Ser503
Accession # O15146
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse MuSK by Western Blot Sorbs1 is enriched at AChR aggregates, and Sorbs1 RNAi blocks AChR clustering in vitro. (A) Treatment of myotubes with siRNA directed against Sorbs1 blocks AChR clustering in C2C12 cells. Montages containing sixteen fields at a magnification of 10× were analyzed with ImageJ software (NIH). (B) Sorbs1 siRNA significantly reduces Sorbs1 protein expression in myotubes. (C) Sorbs1 protein is highly enriched at sites where AChRs aggregate. (D) Agrin stimulates tyrosine phosphorylation of MuSK and Dok-7 at similar levels in myotubes treated with Sorbs1 siRNA. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26527617), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MuSK
MuSK (muscle-specific kinase) is a 100 kDa type I transmembrane (TM) protein belonging to the receptor tyrosine kinase family. It is found in the postsynaptic membrane of skeletal muscle motor endplates. MuSK does not bind agrin directly, but is part of an agrin receptor complex that promotes the clustering of acetylcholine receptor at the neuromuscular junction (NMJ). Over residues 22‑503, human MuSK shares 92% amino acid sequence identity with mouse MuSK. Human MuSK apparently has multiple isoforms. One contains deletions at residues 307‑394 and 454‑461, while a second is a short soluble form that contains residues 120‑209 plus a unique 24 aa C‑terminal tail.
Product Datasheets
Citations for Human MuSK Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Dok-7 regulates neuromuscular synapse formation by recruiting Crk and Crk-L.
Authors: Hallock PT, Xu CF, Park TJ
Genes Dev., 2010-11-01;24(21):2451-61.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Sorbs1 and -2 Interact with CrkL and Are Required for Acetylcholine Receptor Cluster Formation.
Authors: Hallock P, Chin S, Blais S, Neubert T, Glass D
Mol Cell Biol, 2015-11-02;36(2):262-70.
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C2C12 differentiated myotubes, following IP with an anti-MuSK antibody (different from this Ab), SDS-PAGE, and transferred into PVDF membrane. After blocking with 5% BSA/TBST, blot was probed with 1:400 of AF562 (4C, o/n). Subsequent probing with a HRP-anti-goat secondary Ab yielded excellent publishable results, with 100 kDa MuSK band visible.
Differentiated C2C12 mouse skeletal muscle lysates IP'ed for MuSK using a donated Ab. IP works well and I can detect phosphorylation of MuSK using a general phosphotyrosine Ab, but upon stripping, blocking, and probing with this Ab, I am unable to detect MuSK (perhaps due to high background signal). Probing with a different MuSK Ab is much cleaner
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