Human MyD88 Antibody

Catalog #: MAB2928 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2928
MAB2928-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MyD88 Antibody Summary

Species Reactivity

Human

Specificity

Detects human MyD88 in direct ELISAs. Does not cross-react with mouse MyD88.

Source

Monoclonal Rat IgG_2A Clone # 316603

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human MyD88
Met1-Pro296
Accession # Q99836

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

3-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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MyD88 in Raji Human Cell Line. MyD88 was detected in immersion fixed Raji human Burkitt's lymphoma cell line using Rat Anti-Human MyD88 Monoclonal Antibody (Catalog # MAB2928) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MyD88

MyD88 was first identified as a myeloid differentiation primary response protein. It contains a cytoplasmic Toll/interleukin 1 receptor (TIR) domain that classifies it as a member of the TIR superfamily. MyD88 is expressed in many adult tissues including heart, kidney and liver tissue and in monocyte, T, B, NK and dendritic cells.

Long Name

Myeloid Differentiation Primary Response Gene 88

Entrez Gene IDs

4615 (Human); 17874 (Mouse)

Alternate Names

MyD88; MYD88D; myeloid differentiation primary response gene (88); myeloid differentiation primary response protein MyD88