Human MYH6 Antibody Summary
Accession # P13533
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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MYH6 in Human Heart. MYH6 was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human MYH6 Monoclonal Antibody (Catalog # MAB8979) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of cardiomyocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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MYH6 in BG01V Human Embryonic Stem Cells. MYH6 was detected in immersion fixed BG01V human embryonic stem cells differentiated into cardiomyocytes using Mouse Anti-Human MYH6 Monoclonal Antibody (Catalog # MAB8979) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
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Detection of Human MYH6 by Western Blot. Western blot shows lysates of human heart atrium. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human MYH6 Monoclonal Antibody (Catalog # MAB8979) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MYH6 at approximately 230 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MYH6
The MYH6 gene encodes the alpha chain of cardiac myosin, a muscle motor protein with two regions that bind actin at aa 657-679 and 759-773. Defects in this gene cause atrial septal defect 3, hypertrophic cardiac myopathy and sick sinus syndrome. MYH6 participates in embryonic and adult heart development and muscle contraction. In adult heart, the beta chain isoform predominates in the ventricular tissue, while both isoforms are found in variable proportions in atrial tissue. Force development is much faster in atrial tissue and ATP consumption is much higher.
Product Datasheets
Citations for Human MYH6 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Generation of alphaMHC-EGFP knock-in in human pluripotent stem cell line, SNUe003-A-3 using CRISPR/Cas9-based gene targeting
Authors: HM Lee, AH Kim, S Hwang, J Jung, H Seol, JJ Sung, SM Jeong, YM Choi, JK Jun, HS Kim, J Jang
Stem Cell Research, 2022-04-04;61(0):102779.
Species: Human
Sample Types: Transfected Whole Cells
Applications: ICC -
MAP4K4 Inhibition Promotes Survival of Human Stem Cell-Derived Cardiomyocytes and Reduces Infarct Size In�Vivo
Authors: LR Fiedler, K Chapman, M Xie, E Maifoshie, M Jenkins, PA Golforoush, M Bellahcene, M Noseda, D Faust, A Jarvis, G Newton, MA Paiva, M Harada, DJ Stuckey, W Song, J Habib, P Narasimham, R Aqil, D Sanmugalin, R Yan, L Pavanello, M Sano, SC Wang, RD Sampson, S Kanayagana, GE Taffet, LH Michael, ML Entman, TH Tan, SE Harding, CMR Low, C Tralau-Ste, T Perrior, MD Schneider
Cell Stem Cell, 2019-03-07;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: IHC
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This antibody was diluted 1:50 (10ug/ml) and used for IF staining of human iPSC - derived cardiomyocytes (15 days after initiation of differentiation).
