Human Myosin Heavy Chain APC-conjugated Antibody

Catalog #: IC4470A Datasheet / COA / SDS

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IC4470A

Detection of Myosin Heavy Chain in Three-day Differentiated C2C12 Mouse Cell Line by Flow Cytometry.

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Human Myosin Heavy Chain APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects Myosin Heavy Chain in human, mouse, rat and other mammalian, avian, and amphibian species.

Source

Monoclonal Mouse IgG_2B Clone # MF20

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chicken pectoralis-derived Myosin

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of Myosin Heavy Chain antibody in Three-day Differentiated C2C12 Mouse Cell Line antibody by Flow Cytometry.

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Detection of Myosin Heavy Chain in Three-day Differentiated C2C12 Mouse Cell Line by Flow Cytometry. Three-day differentiated C2C12 mouse myoblast cell line was stained with Mouse Anti-Human Myosin Heavy Chain APC-conjugated Monoclonal Antibody (Catalog # IC4470A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Myosin Heavy Chain

Skeletal muscle Myosin or Myosin II is the motor protein that generates force to drive muscle contraction. It is a 520 kDa hexamer comprised of two heavy chains and four light chains. Myosin heavy chain is 220 kDa in size and consists of a long coiled-coil domain tail that mediates dimerization of the two heavy chains and a globular head region that mediates ATP-dependent sliding of Actin filaments. Myosin heavy chain can be proteolytically cleaved to produce heavy Meromyosin, which includes the S1 motor domain (head region) and first third of the coiled-coil domain, and light Meromyosin, which includes the C-terminal two thirds of the coiled-coil domain.

Alternate Names

DFNA4; FLJ13881; FLJ43092; KIAA2034DKFZp667A1311; MHC16; MYH14 variant protein; MYH17; MYHC; Myosin heavy chain 14; Myosin Heavy Chain; Myosin heavy chain, non-muscle IIc; myosin; myosin, heavy chain 14; myosin, heavy chain 14, non-muscle; myosin, heavy polypeptide 14; myosin-14; NMHC II-C; NMHC-II-C; Non-muscle myosin heavy chain IIc; nonmuscle myosin heavy chain II-C