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Human NCAM-1/CD56 Antibody

Catalog #: MAB24081
4 Citations Datasheet / COA / SDS
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MAB24081
MAB24081-SP
Detection of Human NCAM‑1/CD56 by Western Blot.
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Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NCAM-1/CD56 Antibody Summary

Species Reactivity
Human
Specificity
Detects human NCAM-1/CD56 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) ALCAM, rhBCAM, rhEPCAM, rhMCAM, rhNCAM-1-L1, recombinant mouse (rm) MAdCAM-1, rmNCAM-1-L1, or rmOCAM is observed.
Source
Monoclonal Mouse IgG2B Clone # 301021
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human NCAM-1/CD56
Leu20-Pro603 and Glu636-Asn741
Accession # NP_001070150
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
Human brain (cerebellum and hypothalamus)
Flow Cytometry
0.25 µg/106 cells
Human peripheral blood mononuclear cells
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot View Larger

Detection of Human NCAM‑1/CD56 by Western Blot. Western blot shows lysates of human brain (cerebellum and hypothalamus). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human NCAM‑1/CD56 Monoclonal Antibody (Catalog # MAB24081) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for NCAM‑1/CD56 at approximately 105-130 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Flow Cytometry View Larger

Detection of NCAM-1/CD56 in Human PBMC by Flow Cytometry. Human PBMCs were stained with either (A) Mouse Anti-Human NCAM-1/CD56 Monoclonal Antibody (Catalog # MAB24081) or (B) Mouse IgG2B Isotype Control (MAB0041) followed by Anti-Mouse IgG APC-conjugated secondary antibody (F0101B) and Mouse Anti-Human NKp46 PE-conjugated Monoclonal Antibody (FAB1850P). Staining was performed using our Staining Membrane-associated Proteins protocol.

Flow Cytometry View Larger

Detection of NCAM-1/CD56 in Human NK cells by Flow Cytometry. Human NK cells were expanded from PBMCs using Cloudz Human NK Cell Expansion Kit (CLD004) and were stained with either (A) Mouse Anti-Human NCAM-1/CD56 Monoclonal Antibody (Catalog # MAB24081) or (B) Mouse IgG2B Isotype Control (MAB0041) followed by Anti-Mouse IgG APC-conjugated secondary antibody (F0101B) and Mouse Anti-HumanCD3 PE-conjugated Monoclonal Antibody (FAB100P). Staining was performed using our Staining Membrane-associated Proteins protocol.

Immunohistochemistry NCAM‑1/CD56 antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

NCAM‑1/CD56 in Human Brain. NCAM-1/CD56 was detected in immersion fixed paraffin-embedded sections of human brain using Mouse Anti-Human NCAM-1/CD56 Monoclonal Antibody (Catalog # MAB24081) at 25 µg/mL over-night at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NCAM-1/CD56

Neural cell adhesion molecule 1 (NCAM-1) is a multifunctional member of the Ig superfamily. It belongs to a family of membrane-bound glycoproteins that are involved in Ca++ independent cell matrix and homophilic or heterophilic cell-cell interactions. NCAM-1 specifically binds to heparan sulfate proteoglycans (1), the extracellular matrix protein agrin (2), and several chondroitin sulfate proteoglycans that include neurocan and phosphocan (3). There are three main forms of human NCAM-1 that arise by alternate splicing. These are designated NCAM-120/NCAM-1 (761 amino acids [aa]), NCAM-140 (848 aa), and NCAM-180 (1120 aa). NCAM-120 is GPI-linked, while NCAM-140 and NCAM-180 are type I transmembrane glycoproteins (4‑6). Additional alternate splicing adds considerable diversity to all three forms, and extracellular proteolytic processing is possible for NCAM-180 (7‑8). NCAM-1 is synthesized as a 761 aa preproprecursor that contains a 19 aa signal sequence, a 722 aa GPI-linked mature region, and a 20 aa C-terminal prosegment (4). The molecule contains five C-2 type Ig-like domains and two fibronectin type-III domains. Human to mouse, NCAM-1 is 93% aa identical. NCAM-1 appears to be highly sialylated. The polysialyation of NCAM-1 reduces its adhesive property and increases its neurite outgrowth promoting features (9). NCAM-1 in the adult brain shows a decline of sialylation relative to earlier developmental periods. In regions that retain a high degree of neuronal plasticity, however, the adult brain continues to express polysialylation-NCAM-1, suggesting sialylation of NCAM-1 is involved in regenerative processes and synaptic plasticity (10‑13).

References
  1. Burg, M.A. et al. (1995) J. Neurosci. Res. 41:49.
  2. Storms, S.D. and U. Rutishauser (1998) J Biol. Chem. 273:27124.
  3. Margolis, R.K. et al. (1996) Perspect. Dev. Neurobiol. 3:273.
  4. Dickson, G. et al. (1987) Cell 50:1119.
  5. Lanier, L.L. et al. (1991) J. Immunol. 146:4421.
  6. Hemperly, J.J. et al. (1990) J. Mol. Neurosci. 2:71.
  7. Rutishauser, U.and C. Goridis (1986) Trends Genet. 2:72.
  8. Vawter, M.P. et al. (2001) Exp. Neurol. 172:29.
  9. Rutihauser, U. (1990) Adv. Exp. Med. Biol. 265:179.
  10. Becker, C.G. et al. (1996) J. Neurosci. Res. 45:143.
  11. Doherty, P. et al. (1995) J. Neurobiol. 26:437.
  12. Eckardt, M. et al. (2000) J. Neurosci. 20:5234.
  13. Muller, D. et al. (1996) Neuron 17:413.
Long Name
Neural Cell Adhesion Molecule
Entrez Gene IDs
4684 (Human); 17967 (Mouse); 24586 (Rat)
Alternate Names
CD56 / NCAM-1; CD56 antigen; CD56; MSK39; NCAM1; N-CAM-1; NCAM-1; NCAMantigen recognized by monoclonal 5.1H11; neural cell adhesion molecule 1; neural cell adhesion molecule, NCAM

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Citations for Human NCAM-1/CD56 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Diminished expression of major histocompatibility complex facilitates the use of human induced pluripotent stem cells in monkey
    Authors: X Wang, M Lu, X Tian, Y Ren, Y Li, M Xiang, S Chen
    Stem Cell Res Ther, 2020-08-03;11(1):334.
    Species: Human
    Sample Types: Protein
  2. Radiotherapy and chemotherapy change vessel tree geometry and metastatic spread in a small cell lung cancer xenograft mouse tumor model
    Authors: T Frenzel, B Hoffmann, R Schmitz, A Bethge, U Schumacher, G Wedemann
    PLoS ONE, 2017-11-06;12(11):e0187144.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. Selectins mediate small cell lung cancer systemic metastasis.
    Authors: Heidemann F, Schildt A, Schmid K, Bruns O, Riecken K, Jung C, Ittrich H, Wicklein D, Reimer R, Fehse B, Heeren J, Luers G, Schumacher U, Heine M
    PLoS ONE, 2014-04-03;9(4):e92327.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Flow Cytometry
  4. Satellite cells senescence in limb muscle of severe patients with COPD.
    Authors: Theriault ME, Pare MÈ, Maltais F, Debigare R
    PLoS ONE, 2012-06-13;7(6):e39124.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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