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Human Neuronal Pentraxin R/NPTXR Antibody

Catalog #: AF4414
4 Citations Datasheet / COA / SDS
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AF4414
AF4414-SP
Detection of Human Neuronal Pentraxin R/NPTXR by Simple WesternTM.
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Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Neuronal Pentraxin R/NPTXR Antibody Summary

Species Reactivity
Human
Specificity
Detects human Neuronal Pentraxin R/NPTXR in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) NPTX2 and rhNPTX1 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Pentraxin R/NPTXR
Ala24-Lys499
Accession # O95502
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Neuronal Pentraxin R/NPTXR
Simple Western
20 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
Immersion fixed paraffin-embedded sections of Hippocampus

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Simple Western Detection of Human Neuronal Pentraxin R/NPTXR antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human Neuronal Pentraxin R/NPTXR by Simple WesternTM. Simple Western lane view shows lysates of U-87 MG human glioblastoma/astrocytoma cell line and human hippocampus tissue, loaded at 0.2 mg/mL. A specific band was detected for Neuronal Pentraxin R/NPTXR at approximately 66 kDa (as indicated) using 20 µg/mL of Sheep Anti-Human Neuronal Pentraxin R/NPTXR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4414) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunohistochemistry View Larger

Detection of Neuronal Pentraxin R/NPTXR in Hippocampus. Neuronal Pentraxin R/NPTXR was detected in immersion fixed paraffin-embedded sections of Hippocampus using Sheep Anti-Human Neuronal Pentraxin R/NPTXR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4414) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neuronal Pentraxin R/NPTXR

NPTXR (Neuronal Pentraxin Receptor; also NPR) is a 65 kDa, type II transmembrane glycoprotein that binds Neuronal Pentraxins 1 and 2. It is expressed on cerebellar Purkinje and granule cells, and hippocampal neurons of the CA1, CA3 and dentate gyrus regions. Human NPTXR is 499 amino acids (aa) in length. It contains a two aa cytoplasmic tail, a 21 aa transmembrane segment, and a 476 aa extracellular region with one Pentraxin domain (aa 295‑499). One alternate start site exists at Met12, and a 55 kDa soluble form (possibly beginning at Gln56) may be generated by proteolytic cleavage. Over aa 24‑499, human NPTXR is 87% aa identical to mouse NPTXR.

Entrez Gene IDs
23467 (Human); 73340 (Mouse); 81005 (Rat)
Alternate Names
Neuronal Pentraxin R; neuronal pentraxin receptor; NPR; NPTXR

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Citations for Human Neuronal Pentraxin R/NPTXR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. A biomarker-authenticated model of schizophrenia implicating NPTX2 loss of function
    Authors: MF Xiao, SE Roh, J Zhou, CC Chien, BP Lucey, MT Craig, LN Hayes, JM Coughlin, FM Leweke, M Jia, D Xu, W Zhou, C Conover Ta, DB Arnold, M Staley, C Jiang, IM Reti, A Sawa, KA Pelkey, CJ McBain, A Savonenko, PF Worley
    Science Advances, 2021-11-24;7(48):eabf6935.
    Species: Human
    Sample Types: CSF
    Applications: Western Blot
  2. NPTX2 and cognitive dysfunction in Alzheimer's Disease
    Authors: MF Xiao, D Xu, MT Craig, KA Pelkey, CC Chien, Y Shi, J Zhang, S Resnick, O Pletnikova, D Salmon, J Brewer, S Edland, J Wegiel, B Tycko, A Savonenko, RH Reeves, JC Troncoso, CJ McBain, D Galasko, PF Worley
    Elife, 2017-03-23;6(0):.
    Species: Human
    Sample Types: CSF
    Applications: Western Blot
  3. Presynaptic neuronal pentraxin receptor organizes excitatory and inhibitory synapses
    Authors: Sung-Jin Lee
    J. Neurosci, 2016-12-16;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Neuronal pentraxin 2 supports clear cell renal cell carcinoma by activating the AMPA-selective glutamate receptor-4.
    Authors: von Roemeling C, Radisky D, Marlow L, Cooper S, Grebe S, Anastasiadis P, Tun H, Copland J
    Cancer Res, 2014-06-24;74(17):4796-810.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC

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