Human Neurturin Antibody

Catalog #: MAB387
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB387
MAB387-SP

All Neurturin Antibodies

Product Details

Citations (1)

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Neurturin Antibody Summary

Species Reactivity

Human

Specificity

Detects human Neurturin in direct ELISAs and Western blots. In direct ELISAs, approximately 80% cross-reactivity with recombinant mouse Neurturin is observed and no cross-reactivity with recombinant mouse (rm) Artemin, recombinant human (rh) Cripto-1, recombinant Drosophila DPP, rhGDNF, rhLefty A, rmLefty-1, rhMIS, rmNodal, rmPersephin, rhTGF-beta 1, rhLatent TGF-beta 1, rhTGF-beta 1.2, rhTGF-beta 2 or rhTGF-beta 3 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 126422

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Neurturin
Ala96-Val197
Accession # Q99748

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Neurturin (Catalog # 1297-NE)

Blockade of Receptor-ligand Interaction

In a functional ELISA, 0.03-0.15 µg/mL of this antibody will block 50% of the binding of 50 ng/mL of Recombinant Human Neurturin (Catalog # 1297-NE) to immobilized Recombinant Human GFR alpha -2 Fc Chimera (Catalog # 613‑FR) coated at 1 µg/mL (100 µL/well). At 1 μg/mL, this antibody will block >90% of the binding.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neurturin

Neurturin is a member of the GDNF family of ligands and is distantly related to TGF-beta family ligands. It is a disulfide-linked homodimer that interacts with a receptor complex composed of a high affinity ligand binding component (GFR alpha 1 or GFR alpha 2) and a common signaling component, cRET. Neurturin promotes the survival of a variety of neurons and possibly other cell types.

Entrez Gene IDs

4902 (Human); 18188 (Mouse)

Alternate Names

Neurturin; NRTN; NTN

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human Neurturin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Structure and biophysical characterisation of the human full-length Neurturin-GFRa2 complex - a role for heparan sulfate in signalling
Authors: J Sandmark, G Dahl, L Öster, B Xu, P Johansson, T Akerud, A Aagaard, P Davidsson, JM Bigalke, M Sörhede-Wi, GJ Rainey, RG Roth
J. Biol. Chem., 2018-02-02;0(0):.
Species: Rat
Sample Types: Plasma
Applications: ELISA Development (Detection)