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Human Neurturin Antibody

Catalog #: AF387
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF387
AF387-SP
Neuron Survival in Response to Neurturin and Neutralization by Human Neurturin Antibody.
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Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Neurturin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Neurturin in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse Neurturin is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Neurturin
Ala96-Val197
Accession # Q99748
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Neurturin (Catalog # 387-NE)
Immunohistochemistry
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human brain (cerebellum) subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Neutralization
Measured by its ability to neutralize the effect of Neurturin on dorsal root ganglion neurons from E10 chick embryos. Davies, A. M. (1989) in Neurotrophic Factor Bioassay Using Dissociated Neurons, Nerve Growth Factor. Rush, R. A. (eds): John Willey and Sons, Ltd. 95. The Neutralization Dose (ND50) is typically 0.5-2.0 µg/mL in the presence of 6 ng/mL Recombinant Human Neurturin.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Neuron Survival antibody in Response to Neurturin and Neutralization by Human Neurturin Antibody. View Larger

Neuron Survival in Response to Neurturin and Neutralization by Human Neurturin Antibody. Recombinant Human Neurturin (Catalog # 387-NE) supports the survival of dorsal root ganglion neurons from E10 chick embryos in a dose-dependent manner (orange line), as measured by MTT staining. Neuron survival elicited by Recombinant Human Neurturin (6 ng/mL) is neutralized (green line) by increasing concentrations of Human Neurturin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF387). The ND50 is typically 0.5-2.0 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neurturin

Neurturin (NTN) is a member of the GDNF family of ligands which also includes glial cell-derived neurotrophic factor (GDNF), persephin, and artemin. GDNF family proteins are distant members of the TGF-beta superfamily and contain a conserved seven cysteine motif found in the entire family. Human NTN encodes a 197 amino acid (aa) preproprotein with a 19 aa residue putative signal peptide and a 76 aa pro region. Proteolytic cleavage of the pro-protein occurs at an RXXR consensus sequence. The native protein is a disulfide-linked homodimer with a calculated monomeric mass of about 12.5 kDa. The amino acid sequence of human NTN shares 91% identity to mouse NTN. NTN also shares about 42% similarity with GDNF. The bioactivities of all GDNF family ligands are mediated through a receptor complex composed of a high affinity ligand binding component (GFR alpha 1 - GFR alpha 4) and a common signaling component, cRET (receptor tyrosine kinase). NTN prefers to bind GFR alpha 2 but can also bind GFR alpha 1. NTN can promote the survival of a variety of neurons including sympathetic, sensory, and central nervous system neurons. The wide expression of NTN in both neuronal and non-neuronal tissues suggests that NTN may regulate the development and maintenance of the central and peripheral nervous systems and other systems.

References
  1. Kotzbauer, P. et al. (1996) Nature 384:467.
  2. Baloh, R.H. et al. (1997) Neuron 18:793.
  3. Baloh, R.H. et al. (1998) Neuron 21:1291.
Entrez Gene IDs
4902 (Human); 18188 (Mouse)
Alternate Names
Neurturin; NRTN; NTN

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Citations for Human Neurturin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Glial-cell-derived neuroregulators control type 3 innate lymphoid cells and gut defence
    Nature, 2016-07-13;535(7612):440-3.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Blocking
  2. Neurturin gene therapy improves motor function and prevents death of striatal neurons in a 3-nitropropionic acid rat model of Huntington's disease.
    Authors: Ramaswamy S, McBride JL, Herzog CD, Brandon E, Gasmi M, Bartus RT, Kordower JH
    Neurobiol. Dis., 2007-01-25;26(2):375-84.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  3. Delivery of neurturin by AAV2 (CERE-120)-mediated gene transfer provides structural and functional neuroprotection and neurorestoration in MPTP-treated monkeys.
    Authors: Kordower JH, Herzog CD, Dass B, Bakay RA, Stansell J, Gasmi M, Bartus RT
    Ann. Neurol., 2006-12-01;60(6):706-15.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC

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