Human NF-L Antibody

Name: Human NF-L Antibody
Brand: R&D Systems
SKU: MAB22162
Rating: 5 (1 reviews)

Catalog #: MAB22162 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB22162-100
MAB22162-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NF-L Antibody Summary

Species Reactivity

Human

Specificity

Detects human NF-L in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 985917

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human NF-L
Met1-Asp338
Accession # P07196

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Confirmation of Specificity using RNAscope ISH

Immersion fixed U-251 MG human glioblastoma cell line

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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NF‑L in U‑251 MG Human Cell Line. NF‑L was detected in immersion fixed U‑251 MG human glioblastoma cell line (positive staining) and K562 human chronic myelogenous leukemia cell line (negative staining) using Mouse Anti-Human NF‑L Monoclonal Antibody (Catalog # MAB22162) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunocytochemistry

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NF-L in U-251 MG human glioblastoma cell line using RNAscope^®ISH NF-L mRNA was detected in formalin-fixed U-251 MG human glioblastoma cell line probed with ACD RNAScope^®Probe-Hs-NEFL (Catalog # 468671) and stained using ACD RNAscope^®2.5 HD Detection Reagents-RED (Catalog # 32260). This is an In Situ Hybridization control for NF-L expression.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NF-L

NF-L is a 68 kDa light chain cytoskeletal intermediate filament protein that is expressed in neurons. It associates with the 125 kDa NF-M and the 200 kDa NF-H to form neurofilaments.

Long Name

Neurofilament Protein, Light Chain

Entrez Gene IDs

4747 (Human)

Alternate Names

68 kDa neurofilament protein; CMT2E; NEFL; neurofilament protein, light chain; neurofilament subunit NF-L; Neurofilament triplet L protein; neurofilament, light polypeptide; neurofilament-light; NF68; NF68FLJ53642; NFL; NF-L; NFLlight polypeptide 68kDa