Human/Mouse/Rat NFATC3 Antibody

Catalog #: AF5834 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5834
AF5834-SP

Detection of Human and Rat NFATC3 by Western Blot.

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Human/Mouse/Rat NFATC3 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse and rat NFATC3 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human NFATC3 Asp900-Asp1035 Accession # Q12968

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Rat NFATC3 by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat NFATC3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5834) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for NFATC3 at approximately 145 - 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Background: NFATC3

NFATC3 (Nuclear factor of activated T cells C3; also NFAT4 and NFATx) is a 145-150 kDa member of the NFAT family of transcription factors. NFATC3 is widely expressed being found in DP thymocytes, CD4+ T cells, smooth muscle cells, Schwann cells and mast cells. NFATC3 regulates gene transcription as part of a NFATC transcription complex. It is normally cytoplasmic and phosphorylated. Upon a rise in intracellular Ca +2 dephosphorylation occurs via calcineurin, and NFATC3 enters the nucleus. Human NFATC3 is 1075 amino acids (aa) in length. It contains a calcineurin binding site (aa 109-114), two nuclear localization sites (aa 273-275 and 686-688), one nuclear export site (aa 1032-1041) and an RH domain that binds DNA (aa 433-592). There are multiple isoforms with different tissue expression profiles. Over aa 900-1035, human NFATC3 shares 93% aa identity with mouse NFATC3.

Long Name

Nuclear Factor of Activated T Cells C3

Entrez Gene IDs

4775 (Human); 18021 (Mouse)

Alternate Names

NFAT4; NF-AT4; NFAT4nuclear factor of activated T-cells, cytoplasmic 3; NFATC3; NF-ATc3; NFATX; nuclear factor of activated T-cells c3 isoform IE-Xa; nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3; T cell transcription factor NFAT4; T-cell transcription factor NFAT4