Human NHA2/SLC9B2 Antibody

Catalog #: MAB8404 Datasheet / COA / SDS

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MAB8404

Detection of NHA2/SLC9B2 in HEK293 Human Cell Line Transfected with Human NHA2/SLC9B2 and eGFP by Flow Cytometry.

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Human NHA2/SLC9B2 Antibody Summary

Species Reactivity

Human

Specificity

Detects HEK293 cells transfected with human NHA2/SLC9B2 by Flow Cytometry. Does not detect untransfected or irrelevant transfected HEK293 cells.

Source

Monoclonal Mouse IgG_2B Clone # 896151

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human NHA2/SLC9B2
Met1-Val537
Accession # NP_849155

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of NHA2/SLC9B2 in HEK293 Human Cell Line Transfected with Human NHA2/SLC9B2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human NHA2/SLC9B2 and eGFP was stained with and either (A) Mouse Anti-Human NHA2/SLC9B2 Monoclonal Antibody (Catalog # MAB8404) or (B) Mouse IgG_2BFlow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NHA2/SLC9B2

NHA2/SLC9B2 (Solute Carrier family 9, subfamily B, member 2) is a member of the solute carrier family of multi-pass membrane proteins. Sodium hydrogen antiporters, such as SLC9B2 (NHA2), convert the proton motive force established by the respiratory chain or the F1F0 mitochondrial ATPase into sodium gradients that drive other energy-requiring processes, transduce environmental signals into cell responses, or function in drug efflux. SLC9B2 contributes to organellar volume homeostasis and is required for osteoclast differentiation and bone resorption activity.

Long Name

Sodium/Hydrogen Cation Proton Antiporter 2

Entrez Gene IDs

133308 (Human); 7086 (Mouse); 679958 (Rat)

Alternate Names

FLJ23984; Mitochondrial Na(+)/H(+) exchanger NHA2; mitochondrial sodium/hydrogen exchanger NHA2; Na(+)/H(+) exchanger-like domain-containing protein 2; Na+/H+ exchanger domain containing 2; NHA2; NHA2NHE10; NHE domain-containing protein 2; NHE10; NHEDC2; SLC9B2; Sodium/hydrogen exchanger-like domain-containing protein 2