Human NKG2D/CD314 Alexa Fluor® 700-conjugated Antibody
Human NKG2D/CD314 Alexa Fluor® 700-conjugated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of NKG2D/CD314 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes gated on CD3-cells were stained with Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P) and either (A) Mouse Anti-Human NKG2D/CD314 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB139N) or (B) Mouse IgG1Alexa Fluor 700 Isotype Control (Catalog # IC002N). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: NKG2D/CD314
NKG2D, also known as CD314, is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C‑type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8+ alpha beta T cells, gamma δ T cells, NK cells, and NKT cells. In mouse systems NKG2D also occurs on macrophages. Human ligands for NKG2D include MICA, MICB, and ULBP1, 2, and 3. Expression of NKG2D ligands occurs in epithelial cells, tumor cells and under conditions of stress or infection. NKG2D exists as a disulfide-linked homodimer that delivers an activating signal upon ligand binding. Signaling requires association with an adapter protein. Alternative splicing of the NKG2D mRNA results in isoforms with different cytoplasmic domains that can associate either with DAP12 to deliver a true activating signal or with DAP10 resulting in a costimulatory signal. NKG2D has been implicated in anti-tumor surveillance and the immune response against viral infection.
- Li, P. et al. (2001) Nature Immunol. 2:443.
- Steinle, A. et al. (2001) Immunogenetics 53:279.
- Cosman, D. et al. (2001) Immunity 14:123.
- Cerwenka, A. and L. Lanier (2001) Immunol. Rev. 181:158.
- Wu, J. et al. (1999) Science 285:730.
- Diefenbach, A. et al. (2002) Nature Immunol. 3:1142.
- Gilfillan, S. et al. (2002) Nature Immunol. 3:1150.
- Groh, V. et al. (2001) Nature Immunol. 2:255.
- Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521.
- Diefenbach, A. et al. (2001) Nature 413:165.
- NKG2D and its Ligands (2002) www.RnDSystems.com.
Product Datasheets
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
Citations for Human NKG2D/CD314 Alexa Fluor® 700-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Inhibition of Glucose Uptake Blocks Proliferation but Not Cytotoxic Activity of NK Cells
Authors: LK Picard, E Littwitz-S, H Waldmann, C Watzl
Cells, 2022-11-03;11(21):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Human CD8+ T-cells recognizing peptides from Mycobacterium tuberculosis (Mtb) presented by HLA-E have an unorthodox Th2-like, multifunctional, Mtb inhibitory phenotype and represent a novel human T-cell subset.
Authors: van Meijgaarden K, Haks M, Caccamo N, Dieli F, Ottenhoff T, Joosten S
PLoS Pathog, 2015-03-24;11(3):e1004671.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Transmissible cytotoxicity of multiple myeloma cells by cord blood-derived NK cells is mediated by vesicle trafficking.
Authors: Martin-Antonio B, Najjar A, Robinson S, Chew C, Li S, Yvon E, Thomas M, Mc Niece I, Orlowski R, Munoz-Pinedo C, Bueno C, Menendez P, Fernandez de Larrea C, Urbano-Ispizua A, Shpall E, Shah N
Cell Death Differ, 2014-08-29;22(1):96-107.
Species: Human
Sample Types: Whole Cells
Applications: ICC
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