Human NKG2D/CD314 Antibody

Catalog #: MAB1392 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1392-100
MAB1392-SP

Detection of NKG2D/CD314 in NK cells by Flow Cytometry.

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All NKG2D/CD314 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NKG2D/CD314 Antibody Summary

Species Reactivity

Human

Specificity

Detects human NKG2D/CD314 in direct ELISA.

Source

Monoclonal Mouse IgG_2B Clone # 1046002

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human NKG2D/CD314
Phe78-Val216
Accession # P26718

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

NK cells (PBMC treated with 27 ng/ml Recombinant Human IL-2 Protein (Catalog # 202-IL), 10 ng/ml Recombinant Human IL-12 Protein (Catalog # 219-IL), 10 ng/ml Recombinant Human IL-18/IL-1F4 Protein (Catalog # 9124-IL) and 10 ng/ml Recombinant Human IL-21 Protein (Catalog # 8879-IL) for 15 days) and CD3- CD56+ naïve PBMC cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of NKG2D/CD314 in NK cells by Flow Cytometry. NK cells (PBMC treated with 27 ng/ml Recombinant Human IL-2 Protein (202-IL), 10 ng/ml Recombinant Human IL-12 Protein (219-IL), 10 ng/ml Recombinant Human IL-18/IL-1F4 Protein (9124-IL) and 10 ng/ml Recombinant Human IL-21 Protein (8879-IL) for 15 days) were stained with Rabbit Anti-Human NCAM‑1/CD56 PE‑conjugated Monoclonal Antibody (Catalog # FAB24086P) and either (A) Mouse Anti-Human NKG2D/CD314 Monoclonal Antibody (Catalog # MAB1392) or (B) Rabbit IgG Alexa Fluor® 700-conjugated Antibody (Catalog # IC1051N). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of NKG2D/CD314 in CD3- CD56+ naïve PBMC cells by Flow Cytometry. CD3- CD56+ naïve PBMC were stained with Rabbit Anti-Human NCAM‑1/CD56 PE‑conjugated Monoclonal Antibody (Catalog # FAB24086P) and either (A) Mouse Anti-Human NKG2D/CD314 Monoclonal Antibody (Catalog # MAB1392) or (B) Mouse IgG_2B Isotype Control (Catalog # MAB004) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NKG2D/CD314

NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C‑type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8⁺ alpha beta T cells, gamma δ T cells, NK cells and NKT cells. In mouse systems NKG2D also occurs on macrophages. Human ligands for NKG2D include MICA, MICB, and ULBP1, 2, and 3. Expression of NKG2D ligands occurs in epithelial cells, tumor cells and under conditions of stress or infection. NKG2D exists as a disulfide-linked homodimer that delivers an activating signal upon ligand binding. Signaling requires association with an adapter protein. Alternative splicing of the NKG2D mRNA results in isoforms with different cytoplasmic domains that can associate either with DAP12 to deliver a true activating signal or with DAP10 resulting in a costimulatory signal. NKG2D has been implicated in anti-tumor surveillance and the immune response against viral infection.

References

Li, P. et al. (2001) Nature Immunol. 2:443.
Steinle, A. et al. (2001) Immunogenetics 53:279.
Cosman, D. et al. (2001) Immunity 14:123.
Cerwenka, A. and L. Lanier (2001) Immunol. Rev. 181:158.
Wu, J. et al. (1999) Science 285:730.
Diefenbach, A. et al. (2002) Nature Immunol. 3:1142.
Gilfillan, S. et al. (2002) Nature Immunol. 3:1150.
Groh, V. et al. (2001) Nature Immunol. 2:255.
Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521.
Diefenbach, A. et al. (2001) Nature 413:165.
NKG2D and its Ligands (2002) www.rndsystems.com.

Long Name

Natural Killer G2D

Entrez Gene IDs

22914 (Human); 27007 (Mouse); 102120479 (Cynomolgus Monkey)

Alternate Names

CD314 antigen; CD314; D12S2489E; FLJ17759; FLJ75772; Killer cell lectin-like receptor subfamily K member 1; killer cell lectin-like receptor subfamily K, member 1; KLR; KLRK1; NK cell receptor D; NKG2-D type II integral membrane protein; NKG2D; NKG2-D; NKG2-D-activating NK receptor; NKG2DDNA segment on chromosome 12 (unique) 2489 expressed sequence