Human NKp30/NCR3 Antibody Summary
Leu19-Thr138
Accession # Q05D23
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NKp30/NCR3
NKp30, along with NKp44 and NKp46, constitute a group of receptors termed “Natural Cytotoxicity Receptors” (1). These receptors play a major role in triggering NK‑mediated killing of most tumor cells lines. NKp30 is a type I transmembrane protein having a single extracellular V-like immunoglobulin domain (2). A physical association with the ITAM-bearing accessory protein, CD3 zeta, occurs via a charged residue in the NKp30 transmembrane domain. Ligation of NKp30 with a specific antibody results in phosphorylation of CD3 zeta (3). NKp30 is expressed on both resting and activated NK cells of the CD56dim, CD16+ subset that account for more that 85% of NK cells found in peripheral blood and spleen (4). NKp30 is absent from the CD56bright, CD16- subset that constitutes the majority of NK cells in lymph node and tonsil, however, its expression is up‑regulated in these cells upon IL-2 activation (4). Studies with neutralizing antibodies reveal that NKp30 is partially responsible for triggering lytic activity against several tumor cell types and that it is the main receptor responsible for NK‑mediated lysis of immature dendritic cells (2, 5). The ligand(s) recognized by NKp30 has not been described.
- Moretta, L. and A. Moretta (2004) EMBO J. 23:255.
- Pende, D. et al. (1999) J. Exp. Med. 190:1505.
- Augugliaro, R. et al. (2003) Eur. J. Immunol. 33:1235.
- Ferlazzo, G. et al. (2004) J. Immunol. 172:1455.
- Ferlazzo, G. et al. (2002) J. Exp. Med. 195:343.
Product Datasheets
Citations for Human NKp30/NCR3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Homo-oligomerization of the activating natural killer cell receptor NKp30 ectodomain increases its binding affinity for cellular ligands.
Authors: Herrmann, Julia, Berberich, Hannah, Hartmann, Jessica, Beyer, Steffen, Davies, Karen, Koch, Joachim
J Biol Chem, 2013-11-25;289(2):765-77.
Species: Insect, Moth - Trichoplusia ni (Cabbage Looper)
Sample Types: Cell Culture Supernates, Cell Lysates, Whole Cells
Applications: ELISA Development, Immunoprecipitation, Western Blot -
The stalk domain and the glycosylation status of the activating natural killer cell receptor NKp30 are important for ligand binding.
Authors: Hartmann J, Tran T, Kaudeer J, Oberle K, Herrmann J, Quagliano I, Abel T, Cohnen A, Gatterdam V, Jacobs A, Wollscheid B, Tampe R, Watzl C, Diefenbach A, Koch J
J Biol Chem, 2012-07-17;287(37):31527-39.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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