Human NKp44/NCR2 Antibody

Catalog #: AF2249 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2249
AF2249-SP

Human NKp44 Antibody Induces IFN-gamma Secretion in Human NK Cells.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NKp44/NCR2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human NKp44/NCR2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) NKp30, rhNKp46, and rhNKp80 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human NKp44/NCR2
Gln22-Pro190, predicted
Accession # O95944

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<1.5 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human NKp44/NCR2 Fc Chimera (Catalog # 2249-NK)

Agonist Activity

0.2-0.6 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Agonist Activity

Human NKp44 Antibody Induces IFN-? Secretion antibody in Human NK Cells.

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Human NKp44 Antibody Induces IFN-gamma Secretion in Human NK Cells. Human NKp44 Antigen Affinity-purified Polyclonal Antibody induces IFN-gamma secretion in human natural killer (NK) cells activated with 1,000 U/mL Recombinant Human IL-2 (Catalog # 202-IL), in a dose-dependent manner, as measured using the Quantikine Human IFN-gamma ELISA Kit (Catalog # DIF50C). The ED₅₀ for this effect is typically 0.2-0.6 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NKp44/NCR2

NKp44, along with NKp30 and NKp46, constitute a group of receptors termed "Natural Cytotoxicity Receptors" (NCR) (1). These receptors are expressed almost exclusively by NK cells and play a major role in triggering NK-mediated killing of most tumor cell lines. No rodent ortholog to NKp44 has been identified. Human NKp44, also known as NCR2, is a 44 kDa type I transmembrane glycoprotein that is characterized by the presence of one extracellular V-like immunoglobulin domain (2). It is synthesized as a 276 amino acid (aa) precursor that contains a 21 aa signal sequence, a 171 aa extracellular region, a 21 aa transmembrane segment and a 63 aa cytoplasmic tail. Alternate splicing in both the cytoplasmic tail and extracellular region generates multiple isoforms of unknown significance. The Ig-like region is unaffected. A physical association with the ITAM-bearing accessory protein, DAP12, occurs via a charged residue in the NKp44 transmembrane domain. Ligation of NKp44 with a specific antibody results in phosphorylation of DAP12 (3) and activation of target cell lysis in a redirected killing assay (4). NKp44 is absent from resting NK cells but is up‑regulated upon activation with IL-2. Activation-induced expression occurs in the CD56^dim CD16⁺ NK subset that accounts for more than 85% of NK cells found in peripheral blood and spleen, as well as the CD56^bright CD16^- NK subset that constitutes the majority of NK cells in lymph node and tonsil (5). Studies with neutralizing antibodies reveal that NKp44 is partially responsible for triggering lytic activity against several tumor cell types (2, 6). Blocking any of the individual NCRs results in partial inhibition of tumor cell lysis, but nearly complete inhibition of lysis is observed if all three receptors are blocked simultaneously (6). NKp44 has also been implicated in recognition of virus-infected cells through its capacity to bind to viral hemagglutinins (7).

References

Moretta, L. and A. Moretta (2004) EMBO J. 23:255.
Cantoni, C. et al. (1999) J. Exp. Med. 189:787.
Augugliaro, R. et al. (2003) Eur. J. Immunol. 33:1235.
Vitale, M. et al. (1998) J. Exp. Med. 187:2065.
Ferlazzo, G. et al. (2004) J. Immunol. 172:1455.
Pende, D. et al. (1999) J. Exp. Med. 190:1505.
Arnon, T. et al. (2001) Eur. J. Immunol. 31:2680.

Long Name

NK Cell Activating Receptor

Entrez Gene IDs

9436 (Human); 102129442 (Cynomolgus Monkey)

Alternate Names

CD336 antigen; CD336; LY95; LY95lymphocyte antigen 95 homolog (activating NK-receptor; NK-p44); lymphocyte antigen 95 (activating NK-receptor; NK-p44); Lymphocyte antigen 95 homolog; natural cytotoxicity triggering receptor 2; Natural killer cell p44-related protein; NCR2; NK cell activating receptor (NKp44); NK cell-activating receptor; NKp44; NK-p44dJ149M18.1