Human NM23-H1 Antibody

Catalog #: MAB6256 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6256
MAB6256-SP

Detection of Human NM23‑H1 by Western Blot.

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All NM23-H1 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NM23-H1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human NM23‑H1 in Western blots. In Western blots, no cross-reactivity with recombinant human NM23-H2 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 642817

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human NM23‑H1
Met1-Glu152
Accession # P15531

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human NM23‑H1 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human NM23-H1 Monoclonal Antibody (Catalog # MAB6256) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human NM23-H1 and recombinant human NM23-H2 (10 ng/lane) were included. A specific band was detected for NM23-H1 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NM23-H1

NM23-H1 (Non-metastatic protein 23 homolog 1; also NDKA) is a 19‑20 kDa member of the NDK family of enzymes. NM23-H1 is ubiquitous in expression and performs multiple functions. It forms disulfide-linked homohexamers, and heterohexamers with NM23-H2, generating a nucleoside diphosphate kinase that catalyzes a phosphoryl transfer from ATP to a nucleoside diphosphate. It also shows His and Ser/Thr protein kinase activity and forms covalent linkages with molecules diverse as p53 and STRAP. It is found both intracellularly and in blood at ng/mL concentrations. Human NM23-H1 is 152 amino acids (aa) in length, contains one NDP kinase domain (aa 5‑134), and shows acetylation at Ala2 and Lys56, plus phosphorylation at Tyr52, Thr94, Ser122, and Ser125. Human NM23-H1 shares 89% aa identity with human 17‑18 kDa NM23-H2 and 94% aa identity with mouse NM23-H1. A second H1 isoform named NM23-H1B with 25 additional aa at the N‑terminus has also been described.

Long Name

Non-metastatic Protein 23 Homolog 1

Entrez Gene IDs

4830 (Human)

Alternate Names

EC 2.7.4.6; GAAD; Granzyme A-activated DNase; Metastasis inhibition factor nm23; NB; NBS; NDK A; NDKA; NDP kinase A; NDPKA; NDPK-A; NM23A; NM23AWD; NM23H1; NM23-H1; NME1; non-metastatic cells 1, protein (NM23A) expressed in; nucleoside diphosphate kinase A; Tumor metastatic process-associated protein