Human NOV/CCN3 Biotinylated Antibody

Catalog #: BAF1640 Datasheet / COA / SDS

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BAF1640

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Human NOV/CCN3 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human NOV/CCN3 in ELISAs and Western blots. In sandwich immunoassays, approximately 7% cross-reactivity with recombinant mouse NOV is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human NOV/CCN3
Thr32-Met357
Accession # P48745

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Purity

Antigen Affinity-purified

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human NOV/CCN3 (Catalog # 1640-NV)

Immunohistochemistry

5-15 µg/mL

See below

Human NOV/CCN3 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Human NOV/CCN3 Antibody (Catalog # AF1640)

Standard: Recombinant Human NOV/CCN3 Protein, CF (Catalog # 1640-NV)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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NOV/CCN3 in Human Prostate. NOV/CCN3 was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human NOV/CCN3 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1640) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NOV/CCN3

NOV, also called CCN3, is one of six CCN (CYR61/CTGF/NOV) secreted proteins which share a common multimodular organization (1-4). NOV/CCN3 contains an N‑terminal IGFBP domain that appears to be non-functional and a vWF type C and thrombospondin type I domain which mediate oligomerization and matrix interactions, respectively (1, 2). The C-terminal cysteine knot domain interacts with several partners, including the matrix protein fibulin 1C (5), Notch-1 (6), and CCN2, with which it may heterodimerize (2). NOV/CCN3 also interacts with the gap junction protein Connexin43 and mediates suppression of proliferation (7). It also binds the calcium binding protein S100A4 and promotes calcium channel activation (8). The 357 amino acid (aa), 44 kDa human NOV/CCN3 shares 80% aa identity with mouse, rat and dog NOV/CCN3, and 78% aa identity with cow NOV/CCN3. NOV/CCN3 also shows 38-50% aa identity with other family members including WISP proteins, except for WISP-2/CCN5 which lacks the cysteine knot (1). NOV/CCN3 is widely expressed developmentally, especially in muscle, endothelium, nervous system, adrenal cortex and chondrocytes (1-4). In transformed cells, a 32 kDa N-terminally truncated form lacks the signal sequence is localized to the nucleus. Truncation allows a C‑terminal nuclear localization sequence to be active (9). Nuclear NOV/CCN3 acts as a transcriptional repressor but promotes proliferation, presumably by interfering with growth control (9). Full length NOV/CCN3 is a secreted matricellular protein which inhibits cell growth. Interaction of NOV/CCN3 with integrins alpha _v beta ₃ and alpha ₅ beta ₁ mediates endothelial cell adhesion, chemotaxis, and promotes angiogenesis (10, 11). Over-expression of NOV/CCN3 downregulates myogenic genes such as MyoD (12).

References

Perbal, B. (2004) Lancet 363:62.
Perbal, B. (2006) Cell Commun. Signal. 4:3.
Martinerie, C. et al. (1994) Oncogene 9:2729.
Snaith, M.R. et al. (1996) Genomics 38:425.
Perbal, B. et al. (1999) Proc. Natl. Acad. Sci. USA 96:869.
Sakamoto, K. et al. (2002) J. Biol. Chem. 277:29399.
Fu, C.T. et al. (2004) J. Biol. Chem. 279:36943.
Li, C.L. et al. (2002) Mol. Pathol. 55:250.
Planque, N. et al. (2006) J. Cell. Biochem. 99:105.
Lin, C.G. et al. (2003) J. Biol. Chem. 278:24200.
Lin, C.G. et al. (2003) J. Biol. Chem. 280:8229.
Calhabeu, F. et al. (2006) Exp. Cell. Res. 312:1876.

Long Name

Nephroblastoma Overexpressed Gene

Entrez Gene IDs

4856 (Human); 18133 (Mouse)

Alternate Names

CCN family member 3; CCN3; CCN3IGF-binding protein 9; IBP-9; IGFBP-9; IGFBP9novH; Insulin-like growth factor-binding protein 9; nephroblastoma overexpressed gene; Nephroblastoma-overexpressed gene protein homolog; NOV; NOVH; protein NOV homolog