Human NPY1R APC-conjugated Antibody

Catalog #: FAB6400A Datasheet / COA / SDS

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FAB6400A

Detection of NPY1R in MCF‑7 Human Cell Line by Flow Cytometry.

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All NPY1R Antibodies

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Human NPY1R APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human NPY1R in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 556153

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human NPY1R
Met1-Ile384
Accession # P25929

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of NPY1R in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human NPY1R APC-conjugated Monoclonal Antibody (Catalog # FAB6400A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: NPY1R

Neuropeptide Y Receptor type 1 (NPY1R) is a 44 kDa (unglycosylated) member of the G protein coupled receptor 1 family. Human NPY1R is 384 amino acids (aa) in length and contains seven transmembrane regions and three potential sites for N-linked glycosylation. In addition, aa 338 is an S-palmitoyl cysteine lipid-binding site and aa 368 is a phosphoserine. Human NPY1R shares 94% and 93% aa sequence identity with mouse and rat NPY1R, respectively. Functionally, NPY1R is a receptor for Neuropeptide Y and Peptide YY.

Long Name

Neuropeptide Y Receptor Y1

Entrez Gene IDs

4886 (Human); 18166 (Mouse)

Alternate Names

neuropeptide Y receptor type 1; neuropeptide Y receptor Y1; NPY1R; NPY1-R; NPYR; NPYY1