Human Occludin Antibody

Catalog #: MAB7074
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7074
MAB7074-SP

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Occludin Antibody Summary

Species Reactivity

Human

Specificity

Detects human Occludin in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 690213

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human Occludin
Accession # Q16625

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Occludin in HUVEC Human Cells. Occludin was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Mouse Anti-Human Occludin Monoclonal Antibody (Catalog # MAB7074) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Occludin

Occludin is an integral membrane protein with an apparent molecular mass of approximately 65 kDa. It is localized exclusively at tight junctions (TJ) of select epithelial and endothelial cells. The protein is 522 amino acids (aa) in length and contains a cytoplasmic N-terminus, four transmembrane domains, and a long COOH‑terminal cytoplasmic domain (domain E) that contains 255 aa. Residues 1-66 make up the first cytoplasmic domain, and that is included in the MARVEL region consisting of aa 57-210. The MARVEL region is a membrane-associating domain that spans aa 67-243. Of note, residues 92-131 are glycine and tyrosine rich. Residues 244-265 constitute the last transmembrane region, with aa 266-522 representing the long cytoplasmic domain termed domain E. At the TJ, Occludin associates with membrane peripheral protein ZO-1 (220 kDa). Human Occludin shares 90% aa sequence identity with mouse Occludin.

Entrez Gene IDs

100506658 (Human); 18260 (Mouse); 83497 (Rat)

Alternate Names

BLCPMG; Occludin; OCLN

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human Occludin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

IL-13 Impairs Tight Junctions in Airway Epithelia
Authors: H Schmidt, P Braubach, C Schilpp, R Lochbaum, K Neuland, K Thompson, D Jonigk, M Frick, P Dietl, OH Wittekindt
Int J Mol Sci, 2019-06-30;20(13):.
Species: Human
Sample Types: Whole Cells
Applications: ICC