Human Orexin B/Hypocretin-2 Antibody

Catalog #: MAB734
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB734
MAB734-SP

2 Images

Product Details

Citations (3)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Orexin B/Hypocretin-2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Orexin B/Hypocretin‑2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Orexin A is observed.

Source

Monoclonal Mouse IgG₁ Clone # 145202

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

KLH-conjugated human Orexin B/Hypocretin‑2 synthetic peptide
Accession # O43612

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

View Larger

Orexin B in Mouse Hypothalamus. Orexin B was detected in perfusion fixed frozen sections of mouse brain (hypothalamus) using Mouse Anti-Human Orexin B Monoclonal Antibody (Catalog # MAB734) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the neuronal processes and synaptic boutons. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Immunohistochemistry

View Larger

Orexin B, p38 alpha, & Integrin beta 1 in Mouse Brainstem. Orexin B, p38a, and Integrin beta 1 were detected in perfusion fixed frozen sections of mouse brainstem using Mouse Anti-Human Orexin B Monoclonal Antibody (red; Catalog # MAB734), Anti-Human/Mouse/Rat p38a Antigen Affinity-purified Polyclonal Antibody (green; Catalog # AF8691), and Anti-Mouse Integrin beta 1 Antigen Affinity-purified Polyclonal Antibody (blue; Catalog # AF2405). Tissue was incubated with primary antibodies overnight at 4 °C. Tissue was stained using NorthernLights™ 493-conjugated Anti-Rabbit IgG Secondary Antibody (green; Catalog # NL006), NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007), and NorthernLights 637-conjugated Anti-Goat IgG Secondary Antibody (blue; Catalog # NL002). The image of Integrin beta 1 is pseudo-colored for presentation. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Orexin B/Hypocretin-2

Orexin A and Orexin B are neuropeptide hormones derived from proteolytic processing of the Orexin precursor protein (Accession # O43612). Orexins are released from the hypothalamus and are involved in the regulation of sleeping patterns, feeding, and metabolism.

Entrez Gene IDs

3060 (Human)

Alternate Names

Hcrt 2; HCRT; Hcrt2; Hypocretin (orexin) neuropeptide precursor; Hypocretin 2; Hypocretin B; Hypocretin precursor; Hypocretin; Hypocretin2; Hypocretin-2; Orexin B; Orexin neuropeptide precursor; Orexin precursor; Orexin; OrexinB; PPORX; PPOX

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Orexin B/Hypocretin-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

Does Orexin B-Binding Receptor 2 for Orexins Regulate Testicular and Epididymal Functions in Normal and Cryptorchid Dogs?
Authors: Caterina Squillacioti, Alessandra Pelagalli, Loredana Assisi, Anna Costagliola, Luc Van Nassauw, Nicola Mirabella et al.
Frontiers in Veterinary Science
Effects of Orexin B on Swine Granulosa and Endothelial Cells
Authors: Francesca Grasselli, Simona Bussolati, Stefano Grolli, Rosanna Di Lecce, Cecilia Dall’Aglio, Giuseppina Basini
Animals (Basel)
Localization of orexin B and receptor 2 for orexins in testicular cytotypes of the camelid alpaca (Vicugna pacos)
Authors: G Liguori, C Squillacio, L Assisi, N Mirabella, E Langella, A Costagliol, A Vittoria
Reprod. Domest. Anim, 2017-02-08;0(0):.
Species: Alpaca
Sample Types: Whole Tissue
Applications: IHC