Human ORF73 HHV8 Antibody

Catalog #: MAB10389 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10389-100
MAB10389-SP

ORF73 HHV8 in Kaposi's Sarcoma Skin Tissue.

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All ORF73 HHV8 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ORF73 HHV8 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ORF73 HHV8 in direct ELISAs.

Source

Monoclonal Mouse IgG Clone # LN53

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Recombinant protein corresponding to the latent nuclear antigen-1 molecule of ORF73 HHV8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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ORF73 HHV8 in Kaposi's Sarcoma Skin Tissue. ORF73 HHV8 was detected in immersion fixed paraffin-embedded sections of Kaposi's Sarcoma skin tissue using Mouse Anti-Human ORF73 HHV8 Monoclonal Antibody (Catalog # MAB10389) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ORF73 HHV8

Lana1 (also known as ORF73 or HHV8) is a multifunctional protein that plays a role in the replication and long-term persistence of the viral episomal genome in dividing cells. Lana1 has been found to be associated with three different diseases observed in AIDS patients; kaposi's sarcoma, primary effusion lymphoma (which is a rare type of non-Hodgkin lymphoma affecting the body cavities) and multicentric Castleman's disease. To date there is much evidence to support a direct role for Lana1 in kaposi's sarcoma.

Long Name

ORF73 of Human Herpesvirus 8

Entrez Gene IDs

4961527 (Viral)

Alternate Names

HHV 8; HHV8GK18_gp81; Human Herpes Virus 8; Kaposi's sarcoma associated herpes virus; KSHV; LANA1; ORF73