Human PARP Antibody

Detection of Human PARP by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human PARP Monoclonal Antibody (Catalog # MAB8095) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PARP at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

PARP in Human Breast. PARP was detected in immersion fixed paraffin-embedded sections of human breast using Mouse Anti-Human PARP Monoclonal Antibody (Catalog # MAB8095) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the nuclei of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human PARP by Simple Western^TM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and HeLa human cervical epithelial carcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for PARP at approximately 125 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human PARP Monoclonal Antibody (Catalog # MAB8095). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western Blot Shows Human PARP Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and PARP knockout HEK293T cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human PARP Monoclonal Antibody (Catalog # MAB8095) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PARP at approximately 120 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.