Human Pax3 Antibody

Catalog #: AF2457
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2457
AF2457-SP

All Pax3 Antibodies

Product Details

Citations (3)

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Pax3 Antibody Summary

Species Reactivity

Human

Specificity

Detects Pax3 in direct ELISAs and Western blots. In Western blots, approximately 2% cross-reactivity with recombinant human (rh) Pax5 and 1% rhPax7, under reducing conditions, is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Pax3
Met1-Ser215
Accession # NP_000429

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Pax3

Immunocytochemistry

5-15 µg/mL

Immersion fixed B16-F1 mouse melanoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Pax3

Pax3 belongs to the family of paired box transcription factors. Pax family proteins typically contain a paired box domain and a paired-type homeodomain. These transcription factors play critical roles during fetal development. There is 98% homology between human and mouse Pax3.

Long Name

Paired Box Gene 3 [Waardenburg Syndrome 1]

Entrez Gene IDs

5077 (Human); 18505 (Mouse)

Alternate Names

CDHS; HUP2; HUP2MGC120384; MGC120381; MGC120382; MGC120383; MGC134778; paired box 3; paired box gene 3 (Waardenburg syndrome 1); paired box homeotic gene 3; paired box protein Pax-3; paired domain gene 3; paired domain gene HuP2; Pax3; Waardenburg syndrome 1; WS1; WS3

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Pax3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Inï¿½vitro atlas of dorsal spinal interneurons reveals Wnt signaling as a critical regulator of progenitor expansion
Authors: S Gupta, R Kawaguchi, E Heinrichs, S Gallardo, S Castellano, I Mandric, BG Novitch, SJ Butler
Oncogene, 2022-07-19;40(3):111119.
Species: Human
Sample Types: Embryoid Bodies
Applications: IHC
Inhibitory Smads differentially regulate cell fate specification and axon dynamics in the dorsal spinal cord.
Authors: Hazen VM, Phan KD, Hudiburgh S, Butler SJ
Dev. Biol., 2011-06-22;356(2):566-75.
Species: Chicken
Sample Types: Whole Tissue
Applications: IHC
A highly enriched niche of precursor cells with neuronal and glial potential within the hair follicle dermal papilla of adult skin.
Authors: Hunt DP, Morris PN, Sterling J, Anderson JA, Joannides A, Jahoda C, Compston A, Chandran S
Stem Cells, 2007-09-27;26(1):163-72.
Species: Human
Sample Types: Whole Cells
Applications: ICC