Human PD-1 Antibody Summary
Met1-Gln167
Accession # Q15116
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human PD‑1 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human PD-1. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human PD-1 Monoclonal Antibody (Catalog # MAB10861) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for PD-1 at approximately 40-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of PD‑1 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with 5 μg/mL PHA for 2 days were stained with Mouse Anti-Human PD-1 Monoclonal Antibody (Catalog # MAB10861) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human CD3e PE-conjugated Monoclonal Antibody (Catalog # FAB100P). Quadrant markers were set based on control antibody staining (Catalog # MAB002).
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B7-H1 Binding to PD-1-transfected HEK293 Human Cell Line is Blocked by Human PD-1 Antibody. In a functional flow cytometry test, biotinylated recombinant human B7-H1 (10 ng/mL, BT156) binds to HEK293 human embryonic kidney cell line transfected with human PD-1 and eGFP. (A) Binding is completely blocked by 2.5 µg/mL of Mouse Anti-Human PD-1 Monoclonal Antibody (Catalog # MAB10861) but not by (B) 2.5 µg/mL of Mouse IgG1 Isotype Control (MAB002). Protein binding was detected by staining cells with Streptavidin-APC (F0050).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PD-1
Programmed Death-1 (PD-1) is a type I transmembrane protein belonging to the CD28/CTLA-4 family of immunoreceptors that mediate signals for regulating immune responses (1). Members of the CD28/CTLA-4 family have been shown to either promote T cell activation (CD28 and ICOS) or downregulate T cell activation (CTLA-4 and PD-1) (2). PD-1 is expressed on activated T cells, B cells, myeloid cells, and on a subset of thymocytes. In vitro, ligation of PD-1 inhibits TCR-mediated T cell proliferation and production of IL-1, IL-4, IL-10, and IFN-gamma. In addition, PD-1 ligation also inhibits BCR mediated signaling. PD-1 deficient mice have a defect in peripheral tolerance and spontaneously develop autoimmune diseases (2, 3). Two B7 family proteins, PD-L1 (also called B7-H1) and PD-L2 (also known as B7-DC), have been identified as PD-1 ligands. Unlike other B7 family proteins, both PD‑L1 and PD‑L2 are expressed in a wide variety of normal tissues including heart, placenta, and activated spleens (4). The wide expression of PD-L1 and PD-L2 and the inhibitor effects on PD-1 ligation indicate that PD-1 might be involved in the regulation of peripheral tolerance and may help prevent autoimmune diseases (2). The human PD-1 gene encodes a 288 amino acid (aa) protein with a putative 20 aa signal peptide, a 148 aa extracellular region with one immunoglobulin-like V‑type domain, a 24 aa transmembrane domain, and a 95 aa cytoplasmic region. The cytoplasmic tail contains two tyrosine residues that form the Immunoreceptor Tyrosine-based Inhibitory Motif (ITIM) and Immunoreceptor Tyrosine-based Switch Motif (ITSM) that are important in mediating PD-1 signaling. Mouse and human PD-1 share approximately 60% aa sequence identity (4).
- Ishida, Y. et al. (1992) EMBO J. 11:3887.
- Nishimura, H. and T. Honjo (2001) Trends in Immunol. 22:265.
- Latchman, Y. et al. (2001) Nature Immun. 2:261.
- Carreno, B.M. and M. Collins (2002) Annu. Rev. Immunol. 20:29.
Product Datasheets
Citation for Human PD-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Radiotherapy Combined with PD-1 Inhibition Increases NK Cell Cytotoxicity towards Nasopharyngeal Carcinoma Cells
Authors: A Makowska, N Lelabi, C Nothbaum, L Shen, P Busson, TTB Tran, M Eble, U Kontny
Cells, 2021-09-17;10(9):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
FAQs
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What is the light chain of Human PD-1 Antibody, Catalog #s MAB10861 and FAB10861, Clone # 913429?
Catalog #s MAB10861 and FAB10861 have a kappa light chain.
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