Human PD-L1/B7-H1 Antibody Summary
Phe19-Thr239
Accession # Q9NZQ7
Applications
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human PD-L1/B7-H1 Monoclonal Antibody (Catalog # MAB1561R).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human PD-L1/B7-H1 DuoSet ELISA Kit (Catalog # DY156) for convenient development of a sandwich ELISA or the Human/Cynomolgus Monkey PD-L1/B7-H1 Quantikine ELISA Kit (Catalog # DB7H10) for a complete optimized ELISA.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human PD-L1/B7-H1 by Western Blot. Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human PD-L1/B7-H1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF156) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PD-L1/B7-H1 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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PD-L1/B7-H1 in Human Colon and Colon Cancer Tissue. PD-L1/B7-H1 was detected in immersion fixed paraffin-embedded sections of normal human colon (left panel) and human colon cancer tissue (right panel) using Goat Anti-Human PD-L1/B7-H1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF156) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Human PD-L1/B7-H1 ELISA Standard Curve. Recombinant Human PD-L1/B7-H1 protein was serially diluted 2-fold and captured by Mouse Anti-Human PD-L1/B7-H1 Monoclonal Antibody (Catalog # MAB1561R) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human PD-L1/B7-H1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF156) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
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Detection of Human PD-L1/B7-H1 by Simple WesternTM. Simple Western lane view shows lysates of HDLM-2 human Hodgkin's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PD-L1/B7-H1 at approximately 72 kDa (as indicated) using 50 µg/mL of Goat Anti-Human PD-L1/B7-H1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF156) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PD-L1/B7-H1
Human B7 homolog 1 (B7-H1), also called programmed cell death 1 ligand 1 (PDCD1L1) and programmed death ligand 1 (PDL1), is a member of the growing B7 family of immune proteins that provide signals for both stimulating and inhibiting T cell activation. Other family members include B7-1, B7-2, B7-H2, PDL2 and B7-H3. B7 proteins are members of the immunoglobulin (Ig) superfamily, their extracellular domains contain 2 Ig-like domains and all members have short cytoplasmic domains. Among the family members, they share about 20-25% amino acid identity. Human and mouse B7-H1 share approximately 70% amino acid sequence identity. B7-H1 has been identified as one of two ligands for programmed death-1 (PD-1), a member of the CD28 family of immunoreceptors. The B7-H1 gene encodes a 290 amino acid (aa) type I membrane precursor protein with a putative 18 aa signal peptide, a 221 aa extracellular domain, a 21 aa transmembrane region, and a 31 aa cytoplasmic domain. Human B7-H1 is constitutively expressed in several organs such as heart, skeletal muscle, placenta and lung, and in lower amounts in thymus, spleen, kidney and liver. B7-H1 expression is upregulated in a small fraction of activated T and B cells and a much larger fraction of activated monocytes. B7-H1 expression is also induced in dendritic cells and keratinocytes after IFN-gamma stimulation. Interaction of B7-H1 with PD-1 results in inhibition of TCR-mediated proliferation and cytokine production. The B7-H1:PD-1 pathway is involved in the negative regulation of some immune responses and may play an important role in the regulation of peripheral tolerance.
- Nishimura, H. and T. Honjo (2001) Trends in Immunology 22:265.
- Freeman, G.J. et al. (2000) J. Exp. Med. 192:1027.
- Latchman, Y. et al. (2001) Nat. Immunol. 2:261.
Product Datasheets
Citations for Human PD-L1/B7-H1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
18
Citations: Showing 1 - 10
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Small Extracellular Vesicles Harboring PD-L1 in Obstructive Sleep Apnea
Authors: Recoquillon, S;Ali, S;Justeau, G;Riou, J;Martinez, MC;Andriantsitohaina, R;Gagnadoux, F;Trzepizur, W;
International journal of molecular sciences
Species: Human hepegivirus
Sample Types: Cell Lysates, Extracellular Vesicles
Applications: Western Blot -
Interference with pathways activated by topoisomerase inhibition alters the surface expression of PD-L1 and MHC I in colon cancer cells
Authors: M Hassan, V Trung, D Bedi, S Shaddox, D Gunturu, C Yates, P Datta, T Samuel
Oncology Letters, 2022-12-09;25(1):41.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
STAG2 regulates interferon signaling in melanoma via enhancer loop reprogramming
Authors: Z Chu, L Gu, Y Hu, X Zhang, M Li, J Chen, D Teng, M Huang, CH Shen, L Cai, T Yoshida, Y Qi, Z Niu, A Feng, S Geng, DT Frederick, E Specht, A Piris, RJ Sullivan, KT Flaherty, GM Boland, K Georgopoul, D Liu, Y Shi, B Zheng
Nature Communications, 2022-04-06;13(1):1859.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Adipose-Tissue-Derived Mesenchymal Stem Cells Mediate PD-L1 Overexpression in the White Adipose Tissue of Obese Individuals, Resulting in T Cell Dysfunction
Authors: A Eljaafari, J Pestel, B Le Maguere, S Chanon, J Watson, M Robert, E Disse, H Vidal
Cells, 2021-10-03;10(10):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Immune checkpoint molecules B7-H6 and PD-L1 co-pattern the tumor inflammatory microenvironment in human breast cancer
Authors: B Cherif, H Triki, S Charfi, L Bouzidi, WB Kridis, A Khanfir, K Chaabane, T Sellami-Bo, A Rebai
Scientific Reports, 2021-04-06;11(1):7550.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Checkpoint inhibition through small molecule-induced internalization of programmed death-ligand 1
Authors: JJ Park, EP Thi, VH Carpio, Y Bi, AG Cole, BD Dorsey, K Fan, T Harasym, CL Iott, S Kadhim, JH Kim, ACH Lee, D Nguyen, BS Paratala, R Qiu, A White, D Lakshminar, C Leo, RK Suto, R Rijnbrand, S Tang, MJ Sofia, CB Moore
Nature Communications, 2021-02-22;12(1):1222.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Human amnion-derived mesenchymal stem cells attenuate xenogeneic graft-versus-host disease by preventing T cell activation and proliferation
Authors: Y Tago, C Kobayashi, M Ogura, J Wada, S Yamaguchi, T Yamaguchi, M Hayashi, T Nakaishi, H Kubo, Y Ueda
Scientific Reports, 2021-01-28;11(1):2406.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
The innate immune effector ISG12a promotes cancer immunity by suppressing the canonical Wnt/&beta-catenin signaling pathway
Authors: R Deng, C Zuo, Y Li, B Xue, Z Xun, Y Guo, X Wang, Y Xu, R Tian, S Chen, Q Liu, J Chen, J Wang, X Huang, H Li, M Guo, X Wang, M Yang, Z Wu, J Wang, J Ma, J Hu, G Li, S Tang, Z Tu, H Ji, H Zhu
Cell. Mol. Immunol., 2020-09-22;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Mechanisms utilized by feline adipose-derived mesenchymal stem cells to inhibit T lymphocyte proliferation
Authors: N Taechangam, SS Iyer, NJ Walker, B Arzi, DL Borjesson
Stem Cell Res Ther, 2019-06-25;10(1):188.
Species: Feline
Sample Types: Whole Cells
Applications: Flow Cytometry -
Immunological Properties of Human Embryonic Stem Cell-Derived Retinal Pigment Epithelial Cells
Authors: M Idelson, R Alper, A Obolensky, N Yachimovic, J Rachmilewi, A Ejzenberg, E Beider, E Banin, B Reubinoff
Stem Cell Reports, 2018-08-16;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Restoration of T Cell function in multi-drug resistant bacterial sepsis after interleukin-7, anti-PD-L1, and OX-40 administration
Authors: LK Thampy, KE Remy, AH Walton, Z Hong, K Liu, R Liu, V Yi, CD Burnham, RS Hotchkiss
PLoS ONE, 2018-06-26;13(6):e0199497.
Species: Human
Sample Types: Whole Cells
Applications: Inhibition -
Blocking the PD-1/PD-L1 pathway in glioma: a potential new treatment strategy
Authors: S Xue, M Hu, V Iyer, J Yu
J Hematol Oncol, 2017-04-07;10(1):81.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
Hepatitis C Virus Induces MDSCs-Like Monocytes through TLR2/PI3K/AKT/STAT3 Signaling
Authors: N Zhai, H Li, H Song, Y Yang, A Cui, T Li, J Niu, IN Crispe, L Su, Z Tu
PLoS ONE, 2017-01-23;12(1):e0170516.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Mesenchymal Stromal Cell Secretion of Programmed Death-1 Ligands Regulates T Cell Mediated Immunosuppression
Stem Cells, 2016-10-26;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Immunoprecipitation -
Soluble co-signaling molecules predict long-term graft outcome in kidney-transplanted patients.
Authors: Melendreras S, Martinez-Camblor P, Menendez A, Bravo-Mendoza C, Gonzalez-Vidal A, Coto E, Diaz-Corte C, Ruiz-Ortega M, Lopez-Larrea C, Suarez-Alvarez B
PLoS ONE, 2014-12-05;9(12):e113396.
Species: Human
Sample Types: Serum
Applications: ELISA Development -
Overexpression of B7-H1 correlates with malignant cell proliferation in pancreatic cancer.
Authors: Song, Xiao, Liu, Junwei, Lu, Yi, Jin, Hongchua, Huang, Dongshen
Oncol Rep, 2013-12-31;31(3):1191-8.
Species: Human
Sample Types: Protein
Applications: Western Blot -
12-O-tetradecanoyl phorbol 13-acetate induces the expression of B7-DC, -H1, -H2, and -H3 in K562 cells.
Authors: Jang BC, Park YK, Choi IH, Kim SP, Hwang JB, Baek WK, Suh MH, Mun KC, Suh SI
Int. J. Oncol., 2007-12-01;31(6):1439-47.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Aberrant regulation of synovial T cell activation by soluble costimulatory molecules in rheumatoid arthritis.
Authors: Wan B, Nie H, Liu A, Feng G, He D, Xu R, Zhang Q, Dong C, Zhang JZ
J. Immunol., 2006-12-15;177(12):8844-50.
Species: Human
Sample Types: Serum
Applications: ELISA Development
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