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Human PDGF R beta Antibody

Catalog #: AF385
32 Citations 1 Review Datasheet / COA / SDS
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AF385
AF385-SP
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PDGF R beta in Human Breast Cancer Tissue.
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Citations (32)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PDGF R beta Antibody Summary

Species Reactivity
Human
Specificity
Detects human PDGF R beta in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human PDGF R beta
Leu33-Phe530 (Glu241Asp)
Accession # P09619
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human PDGF R beta Fc Chimera (Catalog # 385-PR)
Flow Cytometry
2.5 µg/106 cells
BUD‑8 human fibroblast cell line
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Measured by its ability to neutralize PDGF R beta inhibition of PDGF-BB-dependent proliferation in the NR6R‑3T3 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 10-40 µg/mL in the presence of 2 μg/mL Recombinant Human PDGF R beta Fc Chimera and 4 ng/mL Recombinant Human PDGF-BB.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry PDGF R beta antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

PDGF R beta in Human Breast Cancer Tissue. PDGF R beta was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Goat Anti-Human PDGF R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF385) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Neutralization PDGF R beta Inhibition of PDGF-BB-dependent Cell Proliferation and Neutralization by Human PDGF R beta Antibody. View Larger

PDGF R beta Inhibition of PDGF-BB-dependent Cell Proliferation and Neutralization by Human PDGF R beta Antibody. Recombinant Human PDGF R beta Fc Chimera (385-PR) inhibits Recombinant Human PDGF-BB (220-BB) induced proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human PDGF-BB (4 ng/mL) activity elicited by Recombinant Human PDGF R beta Fc Chimera (2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human PDGF R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF385). The ND50 is typically 10-40 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGF R beta

PDGF is a major serum mitogen that can exist as a homo or hetero-dimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA, PDGF‑BB, and PDGF‑AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Where as PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB only with high-affinity. Both PDGF R alpha and PDGF R beta  are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF, and Flt-3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo-and hetero-dimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Recombinant soluble PDGF R beta binds PDGF with high affinity and is potent PDGF antagonist.

References
  1. Heldin, C.H. and L. Claesson-Welsh (1994) in Guidebook to Cytokines and Their Receptors, Nicola, N.A. ed. Oxford University Press, New York, p. 202.
Long Name
Platelet-derived Growth Factor Receptor beta
Entrez Gene IDs
5159 (Human); 18596 (Mouse); 24629 (Rat)
Alternate Names
beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; CD140b antigen; CD140b; EC 2.7.10; EC 2.7.10.1; JTK12; PDGF R beta; PDGFR; PDGFR1; PDGFRB; PDGF-R-beta; platelet-derived growth factor receptor, beta polypeptide

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Citations for Human PDGF R beta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

32 Citations: Showing 1 - 10
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  1. NOTCH3 drives meningioma tumorigenesis and resistance to radiotherapy
    Authors: Choudhury, A;Cady, M;Lucas, C;Najem, H;Phillips, J;Palikuqi, B;Zakimi, N;Joseph, T;Birrueta, J;Chen, W;Bush, N;Hervey-Jumper, S;Klein, O;Toedebusch, C;Horbinski, C;Magill, S;Bhaduri, A;Perry, A;Dickinson, P;Heimberger, A;Ashworth, A;Crouch, E;Raleigh, D;
    bioRxiv
    Species: Human
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  2. Human pericytes degrade diverse alpha-synuclein aggregates
    Authors: BV Dieriks, B Highet, A Alik, T Bellande, TJ Stevenson, V Low, TI Park, J Correia, P Schweder, RLM Faull, R Melki, MA Curtis, M Dragunow
    PLoS ONE, 2022-11-18;17(11):e0277658.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Microglial debris is cleared by astrocytes via C4b-facilitated phagocytosis and degraded via RUBICON-dependent noncanonical autophagy in mice
    Authors: T Zhou, Y Li, X Li, F Zeng, Y Rao, Y He, Y Wang, M Liu, D Li, Z Xu, X Zhou, S Du, F Niu, J Peng, X Mei, SJ Ji, Y Shu, W Lu, F Guo, T Wu, TF Yuan, Y Mao, B Peng
    Nature Communications, 2022-10-24;13(1):6233.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. miR-181a/b downregulation: a mutation-independent therapeutic approach for inherited retinal diseases
    Authors: S Carrella, M Di Guida, S Brillante, D Piccolo, L Ciampi, I Guadagnino, J Garcia Piq, M Pizzo, E Marrocco, M Molinari, G Petrogiann, S Barbato, Y Ezhova, A Auricchio, B Franco, E De Leonibu, EM Surace, A Indrieri, S Banfi
    Embo Molecular Medicine, 2022-10-04;0(0):e15941.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Engagement of the CXCL12-CXCR4 Axis in the Interaction of Endothelial Progenitor Cell and Smooth Muscle Cell to Promote Phenotype Control and Guard Vascular Homeostasis
    Authors: SF Mause, E Ritzel, A Deck, F Vogt, EA Liehn
    International Journal of Molecular Sciences, 2022-01-14;23(2):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Pericytes as mediators of infiltration of macrophages in multiple sclerosis
    Authors: DK Kaushik, A Bhattachar, BM Lozinski, V Wee Yong
    Journal of Neuroinflammation, 2021-12-24;18(1):301.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  7. Loss with ageing but preservation of frontal�cortical capillary pericytes in post-stroke dementia, vascular dementia and Alzheimer's disease
    Authors: R Ding, Y Hase, M Burke, V Foster, W Stevenson, T Polvikoski, RN Kalaria
    Acta neuropathologica communications, 2021-08-02;9(1):130.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  8. Retinal capillary degeneration and blood-retinal barrier disruption in murine models of Alzheimer's disease
    Authors: H Shi, Y Koronyo, DT Fuchs, J Sheyn, K Wawrowsky, S Lahiri, KL Black, M Koronyo-Ha
    Acta Neuropathol Commun, 2020-11-23;8(1):202.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. ShcD Binds DOCK4, Promotes Ameboid Motility and Metastasis Dissemination, Predicting Poor Prognosis in Melanoma
    Authors: E Aladowicz, L Granieri, F Marocchi, S Punzi, G Giardina, PF Ferrucci, G Mazzarol, M Capra, G Viale, S Confalonie, S Gandini, F Lotti, L Lanfrancon
    Cancers (Basel), 2020-11-13;12(11):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Reciprocal Interaction between Vascular Filopodia and Neural Stem Cells Shapes Neurogenesis in the Ventral Telencephalon
    Authors: B Di Marco, EE Crouch, B Shah, C Duman, MF Paredes, C Ruiz de Al, EJ Huang, J Alfonso
    Cell Rep, 2020-10-13;33(2):108256.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  11. Neutrophil extracellular traps released by neutrophils impair revascularization and vascular remodeling after stroke
    Authors: L Kang, H Yu, X Yang, Y Zhu, X Bai, R Wang, Y Cao, H Xu, H Luo, L Lu, MJ Shi, Y Tian, W Fan, BQ Zhao
    Nat Commun, 2020-05-19;11(1):2488.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. A Practical Guide to the Automated Analysis of Vascular Growth, Maturation and Injury in the Brain
    Authors: R Rust, T Kirabali, L Grönnert, B Dogancay, YDP Limasale, A Meinhardt, C Werner, B Laviña, L Kulic, RM Nitsch, C Tackenberg, ME Schwab
    Front Neurosci, 2020-03-20;14(0):244.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  13. The amyloid-beta degradation intermediate Abeta34 is pericyte-associated and reduced in brain capillaries of patients with Alzheimer's disease
    Authors: T Kirabali, S Rigotti, A Siccoli, F Liebsch, A Shobo, C Hock, RM Nitsch, G Multhaup, L Kulic
    Acta Neuropathol Commun, 2019-12-03;7(1):194.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells, Whole Tissue
    Applications: ICC, IHC, Western Blot
  14. Blood brain barrier leakage is not a consistent feature of white matter lesions in CADASIL
    Authors: RM Rajani, J Ratelade, V Domenga-De, Y Hase, H Kalimo, RN Kalaria, A Joutel
    Acta Neuropathol Commun, 2019-11-21;7(1):187.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  15. Reductions in brain pericytes are associated with arteriovenous malformation vascular instability
    Authors: EA Winkler, H Birk, JK Burkhardt, X Chen, JK Yue, D Guo, WC Rutledge, GF Lasker, C Partow, T Tihan, EF Chang, H Su, H Kim, BP Walcott, MT Lawton
    J. Neurosurg., 2018-12-01;0(0):1-11.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  16. Blood-brain barrier-associated pericytes internalize and clear aggregated amyloid-?42 by LRP1-dependent apolipoprotein E isoform-specific mechanism
    Authors: Q Ma, Z Zhao, AP Sagare, Y Wu, M Wang, NC Owens, PB Verghese, J Herz, DM Holtzman, BV Zlokovic
    Mol Neurodegener, 2018-10-19;13(1):57.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  17. Coculture of endothelial progenitor cells and mesenchymal stem cells enhanced their proliferation and angiogenesis through PDGF and Notch signaling
    Authors: T Liang, L Zhu, W Gao, M Gong, J Ren, H Yao, K Wang, D Shi
    FEBS Open Bio, 2017-10-16;7(11):1722-1736.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  18. Transcriptome analysis of PDGFR?+ cells identifies T-type Ca2+ channel CACNA1G as a new pathological marker for PDGFR?+ cell hyperplasia
    Authors: SE Ha, MY Lee, M Kurahashi, L Wei, BG Jorgensen, C Park, PJ Park, D Redelman, KC Sasse, LS Becker, KM Sanders, S Ro
    PLoS ONE, 2017-08-14;12(8):e0182265.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  19. A tissue-specific role for intraflagellar transport genes during craniofacial development
    Authors: EN Schock, JN Struve, CF Chang, TJ Williams, J Snedeker, AC Attia, RW Stottmann, SA Brugmann
    PLoS ONE, 2017-03-27;12(3):e0174206.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  20. Platelet-derived growth factor primes cancer-associated fibroblasts for apoptosis.
    Authors: Rizvi S, Mertens J, Bronk S, Hirsova P, Dai H, Roberts L, Kaufmann S, Gores G
    J Biol Chem, 2014-06-27;289(33):22835-49.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  21. Growth factor receptor-Src-mediated suppression of GRK6 dysregulates CXCR4 signaling and promotes medulloblastoma migration.
    Authors: Yuan, Liangpin, Zhang, Hongying, Liu, Jingbo, Rubin, Joshua B, Cho, Yoon-Jae, Shu, Hui Kuo, Schniederjan, Matthew, MacDonald, Tobey J
    Mol Cancer, 2013-03-05;12(0):18.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  22. PDGFRbeta expression and function in fibroblasts derived from pluripotent cells is linked to DNA demethylation.
    J. Cell. Sci., 2012-02-17;125(0):2276-87.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  23. Regulation of platelet-derived growth factor receptor function by integrin-associated cell surface transglutaminase.
    Authors: Zemskov EA, Loukinova E, Mikhailenko I, Coleman RA, Strickland DK, Belkin AM
    J. Biol. Chem., 2009-04-22;284(24):16693-703.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  24. Inhibition of anastomotic intimal hyperplasia using a chimeric decoy strategy against NFkappaB and E2F in a rabbit model.
    Authors: Miyake T, Aoki M, Morishita R
    Cardiovasc. Res., 2008-05-31;79(4):706-14.
    Species: Rabbit
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  25. Pancreatic stellate cells: partners in crime with pancreatic cancer cells.
    Authors: Vonlaufen A, Joshi S, Qu C, Phillips PA, Xu Z, Parker NR, Toi CS, Pirola RC, Wilson JS, Goldstein D, Apte MV
    Cancer Res., 2008-04-01;68(7):2085-93.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC, Neutralization
  26. PDGF-C and -D induced proliferation/migration of human RPE is abolished by inflammatory cytokines.
    Authors: Li R, Maminishkis A, Wang FE, Miller SS
    Invest. Ophthalmol. Vis. Sci., 2007-12-01;48(12):5722-32.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  27. Vascular endothelial growth factor can signal through platelet-derived growth factor receptors.
    Authors: Ball SG, Shuttleworth CA, Kielty CM
    J. Cell Biol., 2007-04-30;177(3):489-500.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  28. C-KIT, by interacting with the membrane-bound ligand, recruits endothelial progenitor cells to inflamed endothelium.
    Authors: Dentelli P, Rosso A, Balsamo A, Colmenares Benedetto S, Zeoli A, Pegoraro M, Camussi G, Pegoraro L, Brizzi MF
    Blood, 2007-02-08;109(10):4264-71.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  29. Expression of growth factors and growth factor receptor in non-healing and healing ischaemic ulceration.
    Authors: Murphy MO, Ghosh J, Fulford P, Khwaja N, Halka AT, Carter A, Turner NJ, Walker MG
    Eur J Vasc Endovasc Surg, 2006-01-20;31(5):516-22.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  30. Heterodimerization of FGF-receptor 1 and PDGF-receptor-alpha: a novel mechanism underlying the inhibitory effect of PDGF-BB on FGF-2 in human cells.
    Authors: Faraone D, Aguzzi MS, Ragone G, Russo K, Capogrossi MC, Facchiano A
    Blood, 2005-12-01;107(5):1896-902.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  31. Decorin inhibition of PDGF-stimulated vascular smooth muscle cell function: potential mechanism for inhibition of intimal hyperplasia after balloon angioplasty.
    Authors: Nili N, Cheema AN, Giordano FJ, Barolet AW, Babaei S, Hickey R, Eskandarian MR, Smeets M, Butany J, Pasterkamp G, Strauss BH
    Am. J. Pathol., 2003-09-01;163(3):869-78.
    Species: Rabbit
    Sample Types: Cell Lysates
    Applications: Western Blot
  32. Glucose-induced phosphatidylinositol 3-kinase and mitogen-activated protein kinase-dependent upregulation of the platelet-derived growth factor-beta receptor potentiates vascular smooth muscle cell chemotaxis.
    Authors: Campbell M, Allen WE, Silversides JA, Trimble ER
    Diabetes, 2003-02-01;52(2):519-26.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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Human PDGF R beta Antibody
By Anonymous on 04/30/2019
Application: WB Sample Tested: pericytes Species: Human and human pericytes
Western Blot Human PDGF R beta Antibody AF385