Human PEDFR/PNPLA2 Alexa Fluor® 647-conjugated Antibody

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FAB5387R-100UG
R&D Systems Antibodies
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Human PEDFR/PNPLA2 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human PEDF R/PNPLA2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse PEDF R is observed.
Source
Monoclonal Mouse IgG2b Clone # 494702
Immunogen
E. coli-derived recombinant human PEDF R/PNPLA2
Val162-Thr332
Accession # Q96AD5
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
Y‑79 human retinoblastoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PEDFR/PNPLA2

PEDF R (pigment epithelium-derived factor receptor) is also called ATGL (adipose triglyceride lipase), TTS2 (transport-secretion protein 2), iPLA2 zeta (calcium-independent phospholipase A2 zeta) or desnutrin. It is a 54 kDa transmembrane protein that is member 2 of the patatin-like phospholipase domain-containing protein family (gene name PNPLA2). PEDF is highly expressed in adipose tissue, where it catalyzes formation of diacyglycerol from triglyceride. It has also been found in the retina (as a receptor for PEDF), testis, cardiac and skeletal muscle. Human PEDF R shares 85% amino acid (aa) identity with mouse and rat PEDF R within aa 162‑332, the region used as an immunogen.

Long Name
Pigment Epithelium-derived Factor Receptor/Patatin-like Phospholipase Domain-containing Protein 2
Entrez Gene IDs
57104 (Human); 66853 (Mouse); 361676 (Rat)
Alternate Names
Adipose triglyceride lipase; ATGL; ATGL1110001C14Rik; Calcium-independent phospholipase A2; Desnutrin; EC 3.1.1.3; FP17548; IPLA2-zeta; patatin-like phospholipase domain containing 2; patatin-like phospholipase domain-containing protein 2; patatin-like phospholipase domain-containing protein 2-like; PEDF R; PEDFR; PEDF-R; Pigment epithelium-derived factor; PNPLA2; Transport-secretion protein 2; transport-secretion protein 2.2; triglyceride hydrolase; TTS2; TTS2.2; TTS-2.2; TTS2DKFZp667M109

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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