Human Peptide YY Antibody

Catalog #: MAB94561 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB94561-100
MAB94561-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Peptide YY Antibody Summary

Species Reactivity

Human

Specificity

Detects human YY synthetic peptide in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 1011012

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human Peptide YY synthetic peptide

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human Peptide YY Monoclonal Antibody (Catalog # MAB94562).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human Peptide YY ELISA Standard Curve. Recombinant Human Peptide YY protein was serially diluted 2-fold and captured by Mouse Anti-Human Peptide YY Monoclonal Antibody (Catalog # MAB94561) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Peptide YY Monoclonal Antibody (Catalog # MAB94562) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Peptide YY

Peptide YY (PYY), a secretory peptide, is expressed by L cells (enteroendocrine cells) in the small and large intestine, Islet of Langerhans cells, testis, and in some central and peripheral neurons. Because PYY is released from L cells after feeding, it is thought to produce a satiety signal. While the regulation of appetite by PYY and GLP-1 have been studied extensively, recent evidence supports a role for PYY in Islet of Langerhans beta cell survival. PYY secretion is also modulated by gut microbiota, and levels of colonic PYY have reduced in rats with DSS-induced colitis, and in humans with inflammatory bowel disease (IBD), while circulating PYY increased in IBD.

Entrez Gene IDs

5697 (Human); 217212 (Mouse); 287730 (E. faecalis)

Alternate Names

Peptide tyrosine tyrosine; Peptide YY; Peptide-YY; PYY; PYY1; PYY-I