Human Perilipin-3 Antibody

Catalog #: MAB76641 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB76641
MAB76641-SP

Detection of Human Perilipin‑3 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Perilipin-3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Perilipin‑3 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) Perilipin-2 or rhPerilipin-5 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 779103

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Perilipin‑3
Met1-Lys434
Accession # O60664

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.25 µg/mL

Daudi human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, and K562 human chronic myelogenous leukemia cell line

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

Detection of Human Perilipin-3 antibody by Western Blot.

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Detection of Human Perilipin‑3 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human Perilipin-3 Monoclonal Antibody (Catalog # MAB76641) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Perilipin-3 at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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Perilipin‑3 in Human Placenta. Perilipin-3 was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human Perilipin-3 Monoclonal Antibody (Catalog # MAB76641) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of syncytiotrophoblast cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Perilipin-3

M6PRBP1 (mannose 6-phosphate receptor binding protein), gene name PLIN3 (Perilipin-3), also called TIP47 (tail interacting protein of 47 kDa) or PP17 (placental protein 17), is a 47 kDa intracellular transport protein that belongs to the PAT (Perilipin/Adipophilin/TIP47) family of molecules. The 434 amino acid (aa) human Perilipin‑3, which shares approximately 75% aa sequence identity with mouse and rat Perilipin-3, contains a four-helix bundle thought to interact with membranes, and may be phosphorylated and acetylated. A 251 aa form (isoform A) has an alternate start site at aa 184 of the 434 aa form (isoform B). Perilipin-3 is normally found predominantly in the cytoplasm. It is required for uptake of mannose-6 phosphate receptors into endosomes. In the presence of excess lipids, such as in macrophage-derived foam cells, Perilipin-3 may transfer free fatty acids to lipid droplets, promoting their growth.

Entrez Gene IDs

10226 (Human); 66905 (Mouse); 316130 (Rat)

Alternate Names

Cargo selection protein TIP47; M6PRBP1; M6PRBP1MGC2012; Mannose-6-phosphate receptor-binding protein 1; perilipin 3,47 kDa MPR-binding protein; Perilipin3; Perilipin-3; Placental protein 17,47 kDa mannose 6-phosphate receptor-binding protein; PLIN3; PP17; PP17MGC11117; tail-interacting protein, 47 kD; TIP47; TIP47mannose-6-phosphate receptor binding protein 1