Human Peroxiredoxin 1 Antibody

Detection of Human Peroxiredoxin 1 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, A431 human epithelial carcinoma cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 0.1 µg/mL of Human Peroxiredoxin 1 Monoclonal Antibody (Catalog # MAB3488) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for Peroxiredoxin 1 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Peroxiredoxin 1 in MCF‑7 Human Cell Line. Peroxiredoxin 1 was detected in immersion fixed MCF‑7 human breast cancer cell line using Mouse Anti-Human Peroxiredoxin 1 Monoclonal Antibody (Catalog # MAB3488) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Western Blot Shows Human Peroxiredoxin 1 Specificity Using Knockout Cell Line. Western blot shows lysates of U2OS human osteosarcoma cell line and Peroxiredoxin 1 knockout U2OS cell line (KO). Nitrocellulose membrane was probed with 0.5 µg/mL of Mouse Anti-Human Peroxiredoxin 1 Monoclonal Antibody (Catalog # MAB3488) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody. A specific band was detected for Peroxiredoxin 1 at approximately 22 kDa (as indicated) in the parental U2OS cell line, but is not detectable in knockout U2OS cell line. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

Detection of Peroxiredoxin-1/PRDX1 by Immunoprecipitation. Immunoprecipitation was performed on cell lysate of U2OS human osteosarcoma cell line using 1.0 μg of Mouse Anti-Human PRDX1 Monoclonal Antibody (Catalog # MAB3488) pre-coupled to protein G or protein A beads. Immunoprecipitated PRDX1 was detected with a Goat Anti-PRDX1 Polyclonal Antibody. The Ponceau stained transfers of each blot are shown. SM=10% starting material; UB=10% unbound fraction; IP=immunoprecipitated. Image, protocol, and testing courtesy of YCharOS Inc. (ycharos.com).