Human Phospho-c-Jun (S63) Antibody

Name: Human Phospho-c-Jun (S63) Antibody
Brand: R&D Systems
SKU: MAB8930
Rating: 5 (1 reviews)

Catalog #: MAB8930
2 Citations 1 Review Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB8930
MAB8930-SP

Detection of Human Phospho-c‑Jun (S63) by Western Blot.

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Phospho-c-Jun (S63) Antibody Summary

Species Reactivity

Human

Specificity

Detects human c-Jun when phosphorylated at S63 in Western blots.

Source

Recombinant Monoclonal Rabbit IgG Clone # 1018Y

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Phosphopeptide containing the human c-Jun S63 site
Accession # P05412

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Simple Western

5 µg/mL

See below

Immunocytochemistry

1-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Phospho-c‑Jun (S63) by Western Blot. Western blot shows lysates of HEK293T human embryonic kidney cell line untreated (-) or treated (+) with 20 mJ/cm²ultraviolet light (UV) with a 30 minute recovery. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-c-Jun (S63) Monoclonal Antibody (Catalog # MAB8930) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-c-Jun (S63) at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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c‑Jun in HeLa Human Cell Line. c-Jun phosphorylated at S63 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with Anisomycin using Rabbit Anti-Human Phospho-c-Jun (S63) Monoclonal Antibody (Catalog # MAB8930) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western

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Detection of Human Phospho-c‑Jun (S63) by Simple Western^TM. Simple Western lane view shows lysates of HEK293T human embryonic kidney cell line untreated (-) or treated (+) with 20 mJ/cm²ultraviolet light (UV) with a 30 minute recovery, loaded at 0.2 mg/mL. A specific band was detected for Phospho-c‑Jun (S63) at approximately 49 kDa (as indicated) using 5 μg/mL of Rabbit Anti-Human Phospho-c‑Jun (S63) Monoclonal Antibody (Catalog # MAB8930). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: c-Jun

c-Jun is one of three Jun family proteins making up the Activator Protein-1 (AP-1) transcription factor group. c-Jun acts as a positive regulator of cell proliferation and can induce oncogenic transformation.

Long Name

Cellular Repressor of E1A-stimulated Genes/Transcription Factor AP-1

Entrez Gene IDs

3725 (Human)

Alternate Names

Activator protein 1; AP1; AP-1; cJun; c-Jun; enhancer-binding protein AP1; Jun activation domain binding protein; jun oncogene; jun proto-oncogene; JUN; Proto-oncogene c-Jun; transcription factor AP-1; V-jun avian sarcoma virus 17 oncogene homologp39; v-jun sarcoma virus 17 oncogene homolog (avian); v-jun sarcoma virus 17 oncogene homolog

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Phospho-c-Jun (S63) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Autocrine vitamin D signaling switches off pro-inflammatory programs of TH1 cells
Authors: D Chauss, T Freiwald, R McGregor, B Yan, L Wang, E Nova-Lampe, D Kumar, Z Zhang, H Teague, EE West, KM Vannella, MJ Ramos-Beni, J Bibby, A Kelly, A Malik, AF Freeman, DM Schwartz, D Portilla, DS Chertow, S John, P Lavender, C Kemper, G Lombardi, NN Mehta, N Cooper, MS Lionakis, A Laurence, M Kazemian, B Afzali
Nature Immunology, 2021-11-11;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Stem Cell-Induced Inflammation in Cholesteatoma is Inhibited by the TLR4 Antagonist LPS-RS
Authors: M Schürmann, JFW Greiner, V Volland-Th, F Oppel, C Kaltschmid, H Sudhoff, B Kaltschmid
Cells, 2020-01-14;9(1):.
Species: Human
Sample Types: Whole Cells
Applications: ICC