Human Phospho-p27/Kip1 (T157) Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![Detection of Human Phospho-p27/Kip1 (T157) antibody by Western Blot. Detection of Human Phospho-p27/Kip1 (T157) antibody by Western Blot.](https://resources.rndsystems.com/images/datasheets/antibody/p27_AF1555_Western_Blot_5119.jpg)
Detection of Human Phospho-p27/Kip1 (T157) by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF-I (Catalog # 291-G1) for 20 minutes. PVDF membrane was probed with 0.5 µg/mL Human Phospho-p27/Kip1 (T157) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1555) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-p27/Kip1 (T157) was detected at approximately 27 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda-phosphatase (lambda-PPase) for 1 hour. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: p27/Kip1
The cyclin-dependent kinase (cdk) inhibitor p27/Kip1 is a tumor suppressor protein that acts in the nucleus to enforce cell cycle checkpoints. The Akt protein kinase phosphorylates T157, an amino acid residue that lies within a nuclear localization signal for p27, to prevent nuclear localization and thus preclude the growth inhibitory function of p27.
Product Datasheets
Citations for Human Phospho-p27/Kip1 (T157) Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
17
Citations: Showing 1 - 10
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Hypoxia inducible prolyl hydroxylase PHD3 maintains carcinoma cell growth by decreasing the stability of p27.
Authors: Hogel H, Miikkulainen P, Bino L, Jaakkola PM.
Mol. Cancer.
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Mutant AKT1-E17K is oncogenic in lung epithelial cells
Authors: Carmela De Marco, Donatella Malanga, Nicola Rinaldo, Fernanda De Vita, Marianna Scrima, Sara Lovisa et al.
Oncotarget
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Constitutive activation of Pim1 kinase is a therapeutic target for adult T-cell leukemia
Authors: Marcia Bellon, Ling Lu, Christophe Nicot
Blood
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CACYBP Enhances Cytoplasmic Retention of P27Kip1 to Promote Hepatocellular Carcinoma Progression in the Absence of RNF41 Mediated Degradation
Authors: YF Lian, YL Huang, YJ Zhang, DM Chen, JL Wang, H Wei, YH Bi, ZW Jiang, P Li, MS Chen, YH Huang
Theranostics, 2019-10-22;9(26):8392-8408.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Cytoplasmic p27 promotes epithelial-mesenchymal transition and tumor metastasis via STAT3-mediated Twist1 upregulation.
Authors: Zhao D, Besser A, Wander S, Sun J, Zhou W, Wang B, Ince T, Durante M, Guo W, Mills G, Theodorescu D, Slingerland J
Oncogene, 2015-02-16;34(43):5447-59.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation, Western Blot -
SGK3 is an androgen-inducible kinase promoting prostate cancer cell proliferation through activation of p70 S6 kinase and up-regulation of cyclin D1.
Authors: Wang Y, Zhou D, Chen S
Mol Endocrinol, 2014-04-16;28(6):935-48.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
TRIP6 regulates p27 KIP1 to promote tumorigenesis.
Authors: Lin, Victor T, Lin, Vivian Y, Lai, Yun-Ju, Chen, Chen-Sha, Liu, Kang, Lin, Weei-Chi, Lin, Fang-Tsy
Mol Cell Biol, 2013-01-22;33(7):1394-409.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
XB130 mediates cancer cell proliferation and survival through multiple signaling events downstream of Akt.
Authors: Shiozaki A, Shen-Tu G, Bai X, Iitaka D, De Falco V, Santoro M, Keshavjee S, Liu M
PLoS ONE, 2012-08-23;7(8):e43646.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Promotion of cytoplasmic mislocalization of p27 by Helicobacter pylori in gastric cancer.
Authors: Wen S, So Y, Singh K
Oncogene, 2011-08-15;31(14):1771-80.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Homeodomain-interacting Protein Kinase-2 Stabilizes p27kip1 by Its Phosphorylation at Serine 10 and Contributes to Cell Motility.
Authors: Pierantoni GM, Esposito F, Tornincasa M, Rinaldo C, Viglietto G, Soddu S, Fusco A
J. Biol. Chem., 2011-06-29;286(33):29005-13.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Ablation of Akt2 induces autophagy through cell cycle arrest, the downregulation of p70S6K, and the deregulation of mitochondria in MDA-MB231 cells.
Authors: Santi SA, Lee H
PLoS ONE, 2011-01-31;6(1):e14614.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Akt isoform-specific inhibition of MDA-MB-231 cell proliferation.
Authors: Yang W, Ju JH, Lee KM, Shin I
Cell. Signal., 2010-08-02;23(1):19-26.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Knockdown of protein tyrosine phosphatase SHP-1 inhibits G1/S progression in prostate cancer cells through the regulation of components of the cell-cycle machinery.
Authors: Rodriguez-Ubreva FJ, Cariaga-Martinez AE, Cortes MA, Romero-De Pablos M, Ropero S, Lopez-Ruiz P, Colas B
Oncogene, 2009-10-19;29(3):345-55.
Species: Human
Sample Types: Whole Cells
Applications: Western Blot -
Cytoplasmic sequestration of p27 via AKT phosphorylation in renal cell carcinoma.
Authors: Kim J, Jonasch E, Alexander A, Short JD, Cai S, Wen S, Tsavachidou D, Tamboli P, Czerniak BA, Do KA, Wu KJ, Marlow LA, Wood CG, Copland JA, Walker CL
Clin. Cancer Res., 2009-01-01;15(1):81-90.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
Overcoming trastuzumab resistance in HER2-overexpressing breast cancer cells by using a novel celecoxib-derived phosphoinositide-dependent kinase-1 inhibitor.
Authors: Tseng PH, Wang YC, Weng SC, Weng JR, Chen CS, Brueggemeier RW, Shapiro CL, Chen CY, Dunn SE, Pollak M, Chen CS
Mol. Pharmacol., 2006-08-03;70(5):1534-41.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Comprehensive Molecular Characterization of Papillary Renal-Cell Carcinoma
Authors: Cancer Genome Atlas Research Network, W Marston Linehan, Paul T Spellman, Christopher J Ricketts, Chad J Creighton, Suzanne S Fei et al.
New England Journal of Medicine
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Context Specificity in Causal Signaling Networks Revealed by Phosphoprotein Profiling.
Authors: Hill Steven M, Nesser Nicole K, Johnson-Camacho Katie et al.
Cell Systems
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