Human Phospho-PRAS40 (T246) Antibody

Catalog #: MAB68901 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB68901
MAB68901-SP

Detection of Human and Mouse Phospho-PRAS40 (T246) by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Phospho-PRAS40 (T246) Antibody Summary

Species Reactivity

Human

Specificity

Detects human and mouse PRAS40 when phosphorylated at T246.

Source

Recombinant Monoclonal Rabbit IgG Clone # 1211A

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Phosphopeptide containing the human PRAS40 T246 site
Accession # Q96B36

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse Phospho-PRAS40 (T246) by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line and NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/ml Recombinant Human IGF-I (Catalog # 291-G1) for 60 minutes and 100 ng/ml Recombinant Human PDGF-BB (Catalog # 220-BB) for 20 minutes. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human Phospho-PRAS40 (T246) Monoclonal Antibody (Catalog # MAB68901) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-PRAS40 (T246) at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PRAS40

PRAS40 (Proline-rich AKT1 substrate 1), also known as Akt1S1 and p39, is a 40-42 kDa cytoplasmic phosphoprotein that lacks generally recognized structural motifs. It is widely expressed and is considered to be key regulator of mTORC1 (mTOR plus Raptor and G beta L), a complex through which Akt signals into the cell. Through phosphorylation, mTORC1 activity is upregulated by PRAS40. In particular, nonphosphorylated PRAS40 binds to and serves as a negative regulator of mTORC1 activity. Upon Insulin signaling, PRAS40 is phosphorylated on Thr246, Ser221 and Ser183. This causes it to bind 14-3-3 and results in its dissociation from mTORC1, freeing up mTOR to regulate (positively or negatively) protein synthesis. Human PRAS40 is 256 amino acids (aa) in length. It contains one extended Pro-rich region (aa 35-96) plus at least nine utilized Ser/Thr phosphorylation sites. There is one alternative start site at Met131. Over aa 119-256, human PRAS40 shares 93% aa sequence identity with mouse PRAS40.

Long Name

40 kDa Proline-rich Akt1 Substrate

Entrez Gene IDs

84335 (Human); 67605 (Mouse); 292887 (Rat)

Alternate Names

40 kDa; AKT1 substrate 1 (proline-rich); AKT1S1; Lobe; MGC2865; PRAS40; proline-rich AKT1 substrate 1,40 kDa proline-rich AKT substrate